Progress on isolation and short-term ex-vivo culture of highly purified non-apoptotic human intestinal epithelial cells (IEC)
| Autor: | Johannes Grossmann, Kathrin Walther, Martin Steinkamp, Wolf-Kuno Schmautz, Stephan Kiessling, Gerhard Rogler, Frauke Bataille, Monika Artinger, Jürgen Schölmerich, Michael Schultz, Florian Stadler |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Histology
Time Factors Cell division Cell Survival Cell Blotting Western Apoptosis Cell Separation Biology digestive system Pathology and Forensic Medicine HT29 Cells Interferon-gamma Intestinal mucosa medicine Cell Adhesion Humans Anoikis fas Receptor Intestinal Mucosa Cell adhesion Cells Cultured Epithelial Cells Cell Biology General Medicine Flow Cytometry Cell biology medicine.anatomical_structure Cell culture Immunology Ex vivo Cell Division |
| Zdroj: | European journal of cell biology. 82(5) |
| ISSN: | 0171-9335 |
| Popis: | Intestinal epithelial cells (IEC) form the largest surface of the human body and are of pivotal importance to digest and absorb nutrients. Furthermore these cells play a critical role shielding the organism against microorganisms and toxins present in the intestinal lumen. It is therefore not surprising that a large group of researchers take great interest in the study of these cells. However, to date it is a challenge to purify viable primary human intestinal epithelial cells and it has been even more fastidious to maintain IEC in culture ex-vivo as IEC undergo apoptosis within hours due to loss of cell anchorage ('anoikis') following the isolation process. Over recent years the authors aimed to continuously improve the isolation technique for primary IEC, allowing a simple, effective and rapid isolation of highly purified non-apoptotic human IEC. In this study the newly improved method is presented and applied to establish ex-vivo cultures of highly purified, fully viable primary IEC displaying important functional properties, making these cells amenable for ex-vivo research on primary human intestinal epithelial cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect