New corrosion cast media and its ability for SEM and light microscope investigation

Autor: Elza Azmaiparashvili, S. A. Gusev, Irakli Bebiashvili, D Kordzaia, N. Jalabadze, Marom Sareli, Manana Kakabadze, Keti Tsomaia, Leila Patarashvili
Rok vydání: 2020
Předmět:
Zdroj: Microscopy Research and Technique. 83:778-789
ISSN: 1097-0029
1059-910X
DOI: 10.1002/jemt.23468
Popis: SEM of corrosion casts (CC) provides the opportunities to study the vessels and ducts in the phyllogenetic and ontogenetic (age-related) settings, as well as the pathogenesis, compensation, and sanogenesis in different diseases and experimental models. Along with the refinement of SEM CC, the requirements toward casting media (CM) as nontoxicity, low viscosity, quick polymerization, resistance to corrosion solutions, availability, and so on, gradually has developed. We aimed to adapt the sets widely used in dental practice toward the modern requirements to the CC. The following ratio of the components of Protacryl-M and Aycryl-C sets were used for the preparation CM-0.25 g MAYCRYL Powder +0.08 g Benzoyl Peroxide +5.0 ml Protacryl-M liquid component +0.2 Redont Colour (dye concentrate). The obtained solidifying mass was injected in the blood vessels and biliary ducts of the adult Wistar white rats. The SEM of CC of different organs' vascular networks, as well as a biliary tract, reveals that offered CM excellently replicates the forms and branching features of studied tubular structures of all sizes and gives the adequate imprinting of their luminal surfaces. Besides, CM may provide the replication of perivascular spaces and give the casts having no analogous in the appropriate literature. The CM prepared by us perfectly reproduces all possibilities of famous rubbers widely used for the casting of different vascular-ductular structures. Besides, it presents the new implications, which should be implemented in the profound research of the connective-tissue skeleton of different organs.
Databáze: OpenAIRE
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