Diffusion Analysis within Single Nanometric Apertures Reveals the Ultrafine Cell Membrane Organization
| Autor: | Thomas W. Ebbesen, Didier Marguet, Hervé Rigneault, José Dintinger, Pierre-François Lenne, Fabien Conchonaud, Jérôme Wenger, L. Wawrezinieck |
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| Přispěvatelé: | Institut FRESNEL (FRESNEL), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Science et d'ingénierie supramoléculaires (ISIS), Université Louis Pasteur - Strasbourg I-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Wenger, Jérome, Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
[PHYS.PHYS.PHYS-OPTICS] Physics [physics]/Physics [physics]/Optics [physics.optics]
Materials science Biophysics Nanotechnology Fluorescence correlation spectroscopy 02 engineering and technology Diffusion Quantitative Biology::Subcellular Processes Cell membrane 03 medical and health sciences Membrane Microdomains Chlorocebus aethiops Microscopy medicine Animals Diffusion (business) ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences [PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics] Membranes Plasma membrane organization Resolution (electron density) Image Enhancement 021001 nanoscience & nanotechnology Refractometry Spectrometry Fluorescence Membrane medicine.anatomical_structure Microscopy Fluorescence Diffusion process Chemical physics COS Cells 0210 nano-technology |
| Zdroj: | Biophysical Journal Biophysical Journal, 2007, 92, pp.913-919 Biophysical Journal, Biophysical Society, 2007, 92, pp.913-919 HAL |
| ISSN: | 0006-3495 1542-0086 |
| Popis: | We describe the development of a new methodology to probe the plasma membrane organization of living cells at the nanometric scale. Single nanometric apertures in a metallic film limit the observed membrane area below the optical diffraction barrier. The new approach performs fluorescence correlation spectroscopy with increasing aperture sizes and extracts information on the diffusion process from the whole set of data. In particular, transient diffusion regimes are clearly observed when the probed area comes close to the size of the confining structures. First, this strategy allows identification of the mechanism controlling the diffusion of various fluorescent lipid analogs and green fluorescent protein-tagged proteins. Second, it gives an estimate of the characteristic size of the nanometric membrane heterogeneities, allowing a quantitative study of membrane domains such as lipid rafts. Compared to other optical techniques, this method combines the advantages of high spatio-temporal resolution and direct statistical analysis. |
| Databáze: | OpenAIRE |
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