Carpesium abrotanoides (L.) Root as a Potential Source of Natural Anticancer Compounds: Targeting Glucose Metabolism and PKM2/HIF‐1α Axis of Breast Cancer Cells
| Autor: | Chen-Xuan Mei, Dezhao Lu, Jiafan Feng, Cui Wang, Hong Zhao, Jun-Cheng Wang, Xin-Xin Chai, Yifei Le |
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| Rok vydání: | 2019 |
| Předmět: |
Thyroid Hormones
030309 nutrition & dietetics Lactate dehydrogenase A Antineoplastic Agents Breast Neoplasms Asteraceae PKM2 Carbohydrate metabolism urologic and male genital diseases Plant Roots 03 medical and health sciences 0404 agricultural biotechnology medicine Humans Glycolysis education 0303 health sciences education.field_of_study Plant Extracts Chemistry Glucose transporter Membrane Proteins Cancer 04 agricultural and veterinary sciences Hypoxia-Inducible Factor 1 alpha Subunit medicine.disease 040401 food science In vitro Glucose Biochemistry MCF-7 Cells Carrier Proteins Pyruvate kinase Food Science |
| Zdroj: | Journal of Food Science. 84:3825-3832 |
| ISSN: | 1750-3841 0022-1147 |
| Popis: | Carpesium abrotanoides L. (CA) is widely used as a medicinal plant in Asia. The biological activities of the extract from the roots of Carpesium abrotanoides L. (PCA) and its major components were analyzed in this study. PCA was separated and identified with mass spectrometry. Furthermore, we sought to elucidate the anticancer activity of PCA and its mechanisms. PCA exerted its anti-breast cancer activity through inhibiting the expression of glycolysis-related genes, such as glucose transporter 1, lactate dehydrogenase A, and hexokinase 2. Moreover, PCA downregulated the expression of pyruvate kinase M2 and altered its cellular translocation. We also demonstrated PCA is an inhibitor of the PKM2/hypoxia-inducible factor-1α axis, indicating that PCA is potentially useful as an anti-breast cancer agent. PRACTICAL APPLICATION: In this study, the extract from roots of Carpesium abrotanoides Linn. (PCA) was shown to have a noticeable anticancer effect against breast cancer in vitro, and PCA exerts the anticancer activity by regulating glucose metabolism and PKM2 expression. These findings indicate that PCA is a promising agent with practical applications in the development of functional food containing Carpesium abrotanoides L. root extracts. |
| Databáze: | OpenAIRE |
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