Internalisation of Salmonella spp. by Typha latifolia and Cyperus papyrus in vitro and implications for pathogen removal in Constructed Wetlands
| Autor: | Ereck Chakauya, Wilson Parawira, Alexandros I. Stefanakis, Richwell Alufasi, Phiyani Lebea, Walter Chingwaru |
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| Rok vydání: | 2020 |
| Předmět: |
Salmonella
Veterinary medicine 0208 environmental biotechnology Pathogen removal mechanism Cyperus papyrus Wetland 02 engineering and technology 010501 environmental sciences medicine.disease_cause 01 natural sciences Typha latifolia medicine Environmental Chemistry Salmonella spp Waste Management and Disposal Pathogen 0105 earth and related environmental sciences Water Science and Technology geography Typha geography.geographical_feature_category biology Middle income countries food and beverages Internalisation General Medicine Contamination biology.organism_classification 020801 environmental engineering Wastewater |
| Zdroj: | Environmental Technology. 43:949-961 |
| ISSN: | 1479-487X 0959-3330 |
| Popis: | Summarization: Freshwater contamination by enteric pathogens is implicated in the high frequency of diarrhoeal diseases in low to middle income countries, typically due to poor wastewater management. Constructed Wetlands are a cost-effective and sustainable alternative to conventional/mechanical treatment technologies, but the pathogen removal mechanisms in Constructed Wetlands are not fully understood. This study investigated for the first time the internalisation of Salmonella spp. by Typha latifolia and Cyperus papyrus in hydroponic microcosms. Presence of Salmonella spp. within roots, rhizomes and shoots was assayed using agar-based methods over a period of 12 days. Concentration of Salmonella spp. in growth media showed 2.7 and 4.8 log unit reduction with T. latifolia and C. papyrus, respectively, and 1.8 and 6.0 log unit in unplanted units. Salmonella spp. was recovered from root and rhizome tissues of T. latifolia (up to 4.4 logCFU/g) and C. papyrus (up to 3.4 logCFU/g), and the bacteria were highly concentrated in the epidermis and cortex. However, Salmonella spp. was not detected in the stems and leaves of the two plant species. The present study demonstrates for the first time that these macrophytes internalise cells of Salmonella spp., which could be one pathogen removal mechanism employed by wetland plants. Presented on: Environmental Technology |
| Databáze: | OpenAIRE |
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