Insights into the Dynamic Properties of Keratin Intermediate Filaments in Living Epithelial Cells
| Autor: | Frederick W. Flitney, Robert D. Goldman, Miri Yoon, Robert D. Moir, Satya Khuon, Kyeong Han Yoon |
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| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
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Cytochalasin B Movement Dynein Green Fluorescent Proteins Intermediate Filaments squiggles Vimentin macromolecular substances Fibril Antibodies Green fluorescent protein Genes Reporter Keratin Animals Humans Intermediate filament keratin Cells Cultured chemistry.chemical_classification biology tonofibrils Nocodazole Fluorescence recovery after photobleaching Dyneins Epithelial Cells Cell Biology dynamics Cell biology Luminescent Proteins chemistry motility Microscopy Fluorescence Cytoplasm biology.protein Keratins Original Article Energy Metabolism |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | The properties of keratin intermediate filaments (IFs) have been studied after transfection with green fluorescent protein (GFP)-tagged K18 and/or K8 (type I/II IF proteins). GFP-K8 and -K18 become incorporated into tonofibrils, which are comprised of bundles of keratin IFs. These tonofibrils exhibit a remarkably wide range of motile and dynamic activities. Fluorescence recovery after photobleaching (FRAP) analyses show that they recover their fluorescence slowly with a recovery t1/2 of ∼100 min. The movements of bleach zones during recovery show that closely spaced tonofibrils ( |
| Databáze: | OpenAIRE |
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