An investigation of the correlation between the S-glutathionylated GAPDH levels in blood and Alzheimer’s disease progression
| Autor: | Chia Fan Tsai, Cho Chen Hsieh, Wei Jung Chen, Muh-Shi Lin, Kuan Hung Lin, Chen Wei Tsai, Chai Ching Lin |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Physiology Apoptosis Disease Alzheimer's Disease Diagnostic Radiology 0302 clinical medicine Outpatients Blood plasma Medicine and Health Sciences Enzyme-Linked Immunoassays Glyceraldehyde 3-phosphate dehydrogenase Depression (differential diagnoses) Aged 80 and over Multidisciplinary Cell Death biology Radiology and Imaging Neurodegenerative Diseases Blood Proteins Middle Aged Prognosis Glutathione Magnetic Resonance Imaging Body Fluids Blood Neurology Cell Processes Disease Progression Medicine Female Anatomy Research Article Adult medicine.medical_specialty Patients Imaging Techniques Science Tau protein Research and Analysis Methods Blood Plasma 03 medical and health sciences stomatognathic system Alzheimer Disease Diagnostic Medicine Internal medicine Mental Health and Psychiatry medicine Humans Dementia Immunoassays Aged business.industry Case-control study Biology and Life Sciences Cell Biology medicine.disease Health Care 030104 developmental biology Endocrinology Case-Control Studies Immunologic Techniques biology.protein Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) business Protein Processing Post-Translational 030217 neurology & neurosurgery Follow-Up Studies |
| Zdroj: | PLoS ONE PLoS ONE, Vol 15, Iss 5, p e0233289 (2020) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0233289 |
| Popis: | Alzheimer's disease (AD) is a progressive neurodegenerative disease characterized by two aggregates, namely, amyloid-β (Aβ) plaques and neurofibrillary tangles (NFTs) of hyperphosphorylated tau protein (tau-p), which are released into the blood in a very small amount and cannot be easily detected. An increasing number of recent studies have suggested that S-glutathionylated glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is highly correlated with Aβ in patients with AD and that S-glutathionylated GAPDH plays a role as a proapoptotic factor in AD. We found that S-glutathionylated GAPDH is abundant in the blood of AD patients, which is unusual because S-glutathionylated GAPDH cannot exist in the blood under normal conditions. The aim of this study was to further explore the correlation between the S-glutathionylated GAPDH levels in blood plasma and AD progression. As controls, we recruited 191 people without AD, which included 111 healthy individuals and 37 patients with depression and insomnia, in the psychosomatic clinic. Moreover, 47 patients with AD (aged 40-89 years) were recruited at the neurology clinic. The blood S-glutathionylated GAPDH levels in the AD patients were significantly (p < 0.001) higher (752.7 ± 301.7 ng/dL) than those in the controls (59.92 ± 122.4 ng/dL), irrespective of gender and age. For AD diagnosis, the criterion blood S-glutathionylated GAPDH level > 251.62 ng/dL exhibited 95.74% sensitivity and 92.67% specificity. In fact, the individuals aged 70-89 years, namely, 37 patients from the psychosomatic clinic and 42 healthy individuals, showed significant blood S-glutathionylated GAPDH levels (230.5 ± 79.3 and 8.05 ± 20.51 ng/dL, respectively). This finding might indicate neurodegenerative AD progression in psychosomatic patients and suggests that the degree of neuronal apoptosis during AD progression might be sensitively evaluated based on the level of S-glutathionylated GAPDH in blood. |
| Databáze: | OpenAIRE |
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