In vitro effect of the Escherichia coli heat shock regulatory protein on expression of heat shock genes
| Autor: | Herbert Weissbach, Nathan Brot, R VanBogelen, M Bloom, S Skelly, F Neidhardt |
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| Rok vydání: | 1986 |
| Předmět: |
Anti-sigma factors
Sigma Factor Biology Microbiology chemistry.chemical_compound Sigma factor Transcription (biology) RNA polymerase Heat shock protein Operon Gene expression Escherichia coli Heat shock Molecular Biology Heat-Shock Proteins Polymerase fungi DNA-Directed RNA Polymerases Molecular biology Gene Expression Regulation chemistry Chromatography Gel biology.protein bacteria Electrophoresis Polyacrylamide Gel Research Article |
| Zdroj: | Journal of Bacteriology. 166:380-384 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.166.2.380-384.1986 |
| Popis: | In Escherichia coli, the ability to elicit a heat shock response depends on the htpR gene product. Previous work has shown that the HtpR protein serves as a sigma factor (sigma 32) for RNA polymerase that specifically recognizes heat shock promoters (A.D. Grossman, J.W. Erickson, and C.A. Gross Cell 38:383-390, 1984). In the present study we showed that sigma 32 synthesized in vitro could stimulate the expression of heat shock genes. The in vitro-synthesized sigma 32 was found to be associated with RNA polymerase. In vivo-synthesized sigma 32 was also associated with RNA polymerase, and this polymerase (E sigma 32) could be isolated free of the standard polymerase (E sigma 70). E sigma 32 was more active than E sigma 70 with heat shock genes; however, non-heat-shock genes were not transcribed by E sigma 32. The in vitro expression of the htpR gene required E sigma 70 but did not require E sigma 32. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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