| Popis: |
S1. A. CCK-8 assay was used to detect the cytotoxicity of DTX in DTX-resistant PCa cells (PC3/DR and DU145/DR) and their parental sensitive cells (PC3 and DU145). B. The list of hypoxia-related lncRNAs from Pubmed and the top 80 upregulated lncRNAs in PC3/DR cells from RNA-seq (Jiang X, et al. Front Oncol, 2022). The data are presented as the mean ± S.D. of at least three independent experiments. *P < 0.05. S2. A. The relative expression of lincROR was evaluated by qRT-PCR in PC3 and DU145 cells after being transfected with lincROR. B. DU145 cells were treated with different dosages of DTX (concentration of 0, 5, 10, 20, 40, and 80 nM) for 72 hours and cell viability was evaluated by a CCK-8 assay. C. The LDH release in the culture medium was detected in lincROR-transfected DU145 cells after DTX treatment. D. The expression of lincROR was evaluated in PC3/DR and DU145/DR cells after transfected with siROR #1 or #2. E. The cytotoxicity of DTX in DU145/DR cells was detected by CCK-8 assay after lincROR knockdown. F. The LDH release in culture medium was detected in lincROR-knockdown DU145/DR cells after DTX treatment. The data are presented as the mean ± S.D. of at least three independent experiments. |
