A 76-bp deletion in the Mip gene causes autosomal dominant cataract in Hfi mice
| Autor: | Angelika Neuhäuser-Klaus, Chiaki Ohtaka-Maruyama, Patricia A. Grimes, William R. Skach, Walter Pretsch, Dwight Stambolian, Yun Lu, Ana B. Chepelinsky, Peter M.T. Deen, Alvina Bragin, Jack Favor, D.J. Sidjanin, Devonne M. Parker-Wilson |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Genotype Ratón Xenopus Mutant Blotting Western DNA Mutational Analysis Regulation of salt and water reabsorption in the renal collecting duct Gene Expression Biology Aquaporins Cataract Cell Line Exon Mice Gene expression Lens Crystalline Genetics Animals Humans RNA Messenger Eye Proteins Genes Dominant Sequence Deletion Mice Inbred C3H Membrane Glycoproteins Wild type Chromosome Mapping Heterozygote advantage DNA Molecular biology Phenotype Mice Mutant Strains Mice Inbred C57BL Membrane protein Regulatie water en zouttransport in de verzamelbuis van de nier Animals Newborn Mutation Oocytes Female |
| Zdroj: | Genomics : International Journal of Gene Mapping and Nucleotide Sequencing Emphasizing Analyses of the Human and Other Complex Genomes, 74, 313--9 Genomics : International Journal of Gene Mapping and Nucleotide Sequencing Emphasizing Analyses of the Human and Other Complex Genomes, 74, 3, pp. 313--9 |
| ISSN: | 0888-7543 |
| DOI: | 10.1006/geno.2001.6509 |
| Popis: | Item does not contain fulltext Hfi is a dominant cataract mutation where heterozygotes show hydropic lens fibers and homozygotes show total lens opacity. The Hfi locus was mapped to the distal part of mouse chromosome 10 close to the major intrinsic protein (Mip), which is expressed only in cell membranes of lens fibers. Molecular analysis of Mip revealed a 76-bp deletion that resulted in exon 2 skipping in Mip mRNA. In Hfi/Hfi this deletion resulted in a complete absence of the wildtype Mip. In contrast, Hfi/+ animals had the same amount of wildtype Mip as +/+. Results from pulse-chase expression studies excluded hetero-oligomerization of wildtype and mutant Mip as a possible mechanism for cataract formation in the Hfi/+. We propose that the cataract phenotype in the Hfi heterozygote mutant is due to a detrimental gain of function by the mutant Mip resulting in either cytotoxicity or disruption in processing of other proteins important for the lens. Cataract formation in the Hfi/Hfi mouse is probably a combined result of both the complete loss of wildtype Mip and a gain of function of the mutant Mip. |
| Databáze: | OpenAIRE |
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