Comparison of the 'chemical' and 'structural' approaches to the optimization of the thrombin-binding aptamer
| Autor: | Nikolay A. Barinov, Edward N. Timofeev, Igor P. Smirnov, Dmitry V. Klinov, Olga N. Tatarinova, Dmitry N. Kaluzhny, Anna M. Varizhuk, Vladimir B. Tsvetkov, A. N. Chuvilin, Galina E. Pozmogova, Dmitry Basmanov |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Macromolecular Assemblies
Models Molecular Drugs and Devices Drug Research and Development Aptamer Molecular Conformation Thrombin Signaling lcsh:Medicine Electrophoretic Mobility Shift Assay Computational biology Molecular Dynamics Simulation G-quadruplex Microscopy Atomic Force Biochemistry Molecular dynamics Structure-Activity Relationship Nucleic Acids Drug Discovery Molecular Cell Biology Structure–activity relationship Signaling in Cellular Processes Biomacromolecule-Ligand Interactions lcsh:Science Biology Bioinorganic Chemistry Thrombin-binding aptamer Multidisciplinary Oligonucleotide Chemistry lcsh:R Thrombin Chemical modification Aptamers Nucleotide Triazoles Molecular biology G-Quadruplexes Medicine lcsh:Q Biosensor Research Article Biotechnology Signal Transduction |
| Zdroj: | PLoS ONE, Vol 9, Iss 2, p e89383 (2014) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Noncanonically structured DNA aptamers to thrombin were examined. Two different approaches were used to improve stability, binding affinity and biological activity of a known thrombin-binding aptamer. These approaches are chemical modification and the addition of a duplex module to the aptamer core structure. Several chemically modified aptamers and the duplex-bearing ones were all studied under the same conditions by a set of widely known and some relatively new methods. A number of the thrombin-binding aptamer analogs have demonstrated improved characteristics. Most importantly, the study allowed us to compare directly the two approaches to aptamer optimization and to analyze their relative advantages and disadvantages as well as their potential in drug design and fundamental studies. |
| Databáze: | OpenAIRE |
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