In Vitro Antimicrobial Activity and Probiotic Potential of Bifidobacterium and Lactobacillus against Species of Clostridium
| Autor: | Camilla Itapary dos Santos, Monique Santos do Carmo, Silvio Gomes Monteiro, Valério Monteiro-Neto, Cinara R A V Monteiro, Matheus Silva Alves, Bruna de Oliveira de Melo, Elizabeth S. Fernandes, Maria Rosa Quaresma Bomfim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Clostridium perfringens 030106 microbiology lcsh:TX341-641 Microbial Sensitivity Tests medicine.disease_cause Lactobacillus plantarum Article Bacterial Adhesion law.invention Microbiology Clostridium Bile Acids and Salts 03 medical and health sciences Probiotic Anti-Infective Agents law Lactobacillus medicine Humans Clostridium butyricum Bifidobacterium Nutrition and Dietetics antimicrobial activity biology dysbiosis Hydrogen-Ion Concentration Clostridium difficile biology.organism_classification Antimicrobial Gastrointestinal Microbiome 030104 developmental biology probiotics Clostridium Infections Microbial Interactions lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Nutrients Volume 11 Issue 2 Nutrients, Vol 11, Iss 2, p 448 (2019) |
| ISSN: | 2072-6643 |
| DOI: | 10.3390/nu11020448 |
| Popis: | Many Clostridium species are found as commensal members of the intestinal microbiota. However, imbalances of the microbiota may lead to certain infections caused by these microorganisms, mainly Clostridium butyricum, Clostridium difficile, and Clostridium perfringens. In many cases, infection recurrence can occur after antibiotics, indicating the need for novel therapeutic options that act on the pathogens and also restore the microbiota. Herein, the in vitro antimicrobial activity and probiotic potential of clinical and reference strains of Bifidobacterium and Lactobacillus were investigated against Clostridium species. Antimicrobial activity was evaluated by the agar spot test and inhibition of gas production. Then, the probiotic potential of selected strains was assessed by analyzing their coaggregation ability, adhesive properties to host cells and mucin, tolerance to acidic pH and bile salts, and antimicrobial susceptibility profiles. Lactobacillus plantarum ATCC 8014 was the most promising strain based on its inhibitory activity against Clostridium spp. Also, this strain met criteria to be considered a probiotic based on its coaggregation ability, adhesive properties, and tolerance to harsh pH and bile acid salt conditions. The results indicate that among the studied strains, L. plantarum ATCC 8014 presents probiotic potential for controlling infections induced by the studied Clostridium species and should be further evaluated in in vivo animal models. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|