Noradrenaline up-regulates the neuronal and the inducible nitric oxide synthase isoforms in magnocellular neurons of rat brain slices

Autor: Valérie Grange-Messent, Bernard Dugas, André Calas, Danièle Raison
Přispěvatelé: Neurobiologie des signaux intercellulaires (NSI), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Marie, Jean-Luc
Rok vydání: 2004
Předmět:
Male
medicine.medical_specialty
Time Factors
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Nitric Oxide Synthase Type II
Neuropeptide
Nerve Tissue Proteins
Stimulation
Nitric Oxide Synthase Type I
In Vitro Techniques
Biology
Nitric oxide
Norepinephrine
Cellular and Molecular Neuroscience
chemistry.chemical_compound
Internal medicine
Lactation
medicine
Animals
Protein Isoforms
RNA
Messenger
Rats
Wistar
Neurons
Messenger RNA
Microscopy
Confocal
Reverse Transcriptase Polymerase Chain Reaction
[SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Immunohistochemistry
Rats
Nitric oxide synthase
Endocrinology
medicine.anatomical_structure
Gene Expression Regulation
nervous system
chemistry
Hypothalamus
biology.protein
Magnocellular cell
Nitric Oxide Synthase
Supraoptic Nucleus
Paraventricular Hypothalamic Nucleus
Zdroj: Journal of Neuroscience Research
Journal of Neuroscience Research, 2004, pp.690
Journal of Neuroscience Research, Wiley, 2004, pp.690
ISSN: 1097-4547
0360-4012
DOI: 10.1002/jnr.20331
Popis: Nitric oxide (NO) and noradrenaline (NA) are suggested to be implicated in the regulation of neuropeptide secretion in the supraoptic nuclei (SON) and the paraventricular nuclei (PVN) of the hypothalamus. Our study demonstrates short-term interactions between NA and the activity and expression of NO synthase (NOS) in magnocellular neurons, by using an ex vivo model of hypothalamic slices. In the SON as well as in the PVN, total NOS activity exhibited a time-dependant increase after an incubation with NA. In the SON, this increase of total NOS activity was in part the consequence of stimulation of the iNOS activity. Coimmunodetections showed that cells expressing the inducible form of NOS were not astrocytes but magnocellular neurons. Steady-state levels of iNOS and nNOS mRNA were dramatically enhanced by NA, particularly in the SON. Consequently, we provide new evidence that iNOS could play an important role in multiple physiological functions, including extracellular fluid balance, lactation, and parturition.
Databáze: OpenAIRE
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