Topology of Euglena Chloroplast Protein Precursors within Endoplasmic Reticulum to Golgi to Chloroplast Transport Vesicles
| Autor: | Umesh S. Muchhal, Steven D. Schwartzbach, Chidananda Sulli, Zhiwei Fang |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Chloroplasts
Molecular Sequence Data Golgi Apparatus Biology Endoplasmic Reticulum Vesicle lumen Biochemistry symbols.namesake Dogs Chloroplast localization Animals Amino Acid Sequence Protein Precursors Molecular Biology Peptide sequence Integral membrane protein DNA Primers Signal peptidase Base Sequence Sequence Homology Amino Acid Endoplasmic reticulum Biological Transport Cell Biology Golgi apparatus N-terminus symbols Euglena |
| Zdroj: | Journal of Biological Chemistry. 274:457-463 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.274.1.457 |
| Popis: | Euglena chloroplast protein precursors are transported as integral membrane proteins from the endoplasmic reticulum (ER) to the Golgi apparatus prior to chloroplast localization. All Euglena chloroplast protein precursors have functionally similar bipartite presequences composed of an N-terminal signal peptide domain and a stromal targeting domain containing a hydrophobic region approximately 60 amino acids from the predicted signal peptidase cleavage site. Asparagine-linked glycosylation reporters and presequence deletion constructs of the precursor to the Euglena light-harvesting chlorophyll a/b-binding protein of photosystem II (pLHCPII) were used to identify presequence regions translocated into the ER lumen and stop transfer membrane anchor domains. An asparagine-linked glycosylation site present at amino acid 148 of pLHCPII near the N terminus of mature LHCPII was not glycosylated in vitro by canine microsomes while an asparagine-linked glycosylation site inserted at amino acid 40 was. The asparagine at amino acid 148 was glycosylated upon deletion of amino acids 46-146, which contain the stromal targeting domain, indicating that the hydrophobic region within this domain functions as a stop transfer membrane anchor sequence. Protease protection assays indicated that for all constructs, mature LHCPII was not translocated across the microsomal membrane. Taken together with the structural similarity of all Euglena presequences, these results demonstrate that chloroplast precursors are anchored within ER and Golgi transport vesicles by the stromal targeting domain hydrophobic region oriented with the presequence N terminus formed by signal peptidase cleavage in the vesicle lumen and the mature protein in the cytoplasm. |
| Databáze: | OpenAIRE |
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