A Plasmodium falciparum gene coding for a 15-kilodalton antigen expressed in asexual stage parasites, gametocytes and gametes
| Autor: | Jean-Christophe Barale, Thierry Blisnick, Gordon Langsley, David C. Kaslow, Catherine Braun-Breton, T. Ponnudurai, Patricia Barbot |
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| Přispěvatelé: | Parasitologie Expérimentale, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), National Institutes of Health [Bethesda] (NIH), University of Nijmegen, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 1992 |
| Předmět: |
MESH: Introns
Genes Protozoan Antibodies Protozoan Fluorescent Antibody Technique MESH: Amino Acid Sequence MESH: Base Sequence law.invention MESH: Recombinant Proteins law MESH: Animals Genomic library MESH: Fluorescent Antibody Technique ComputingMilieux_MISCELLANEOUS MESH: Plasmodium falciparum Polymerase chain reaction 0303 health sciences biology 030302 biochemistry & molecular biology Exons MESH: Gene Expression Regulation Recombinant Proteins 3. Good health [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology MESH: Genes Protozoan Blotting Western Molecular Sequence Data Plasmodium falciparum MESH: DNA Protozoan Antigens Protozoan Trypanosoma brucei Open Reading Frames 03 medical and health sciences Complementary DNA MESH: Gene Library parasitic diseases Animals MESH: Antibodies Protozoan MESH: Blotting Western Amino Acid Sequence Molecular Biology Gene Gene Library 030304 developmental biology MESH: Molecular Sequence Data Base Sequence DNA Protozoan MESH: Open Reading Frames biology.organism_classification Virology Introns Open reading frame genomic DNA Gene Expression Regulation Parasitology MESH: Exons MESH: Antigens Protozoan |
| Zdroj: | Molecular and Biochemical Parasitology Molecular and Biochemical Parasitology, Elsevier, 1992, 55 (1-2), pp.221-224. ⟨10.1016/0166-6851(92)90143-8⟩ Molecular and Biochemical Parasitology, 1992, 55 (1-2), pp.221-224. ⟨10.1016/0166-6851(92)90143-8⟩ |
| ISSN: | 0166-6851 |
| DOI: | 10.1016/0166-6851(92)90143-8 |
| Popis: | A monoclonal antibody (mAT3) to Trypanosoma brucei glycosyl-phosphatidylinositolphospholipase C (GPI-PLC) which immunoprecipitates Plasmodium falciparum GPI-anchor degrading activities [1,2] was used to screen a 2gtl 1 cDNA expression library. One clone (7.1) was isolated containing a 73-bp insert which was amplified from the 2gtll phage by polymerase chain reaction (PCR) and used to screen asexual stage cDNA, sexual stage cDNA and genomic DNA libraries. In this way the complete gene was obtained and its sequence determined (Fig. 1). A single open reading frame of 372 bp coding for a 15-kDa protein (Agl5) was identified. Comparison of the sexual and asexual stage |
| Databáze: | OpenAIRE |
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