Development of a Biofilm Production-Deficient Escherichia coli Strain as a Host for Biotechnological Applications
| Autor: | Ju Young Lee, Bong Hyun Sung, Mi Sun Kim, Sun Chang Kim, Byung Jo Yu, Frederick R. Blattner, Jun Hyoung Lee, Choong Hoon Lee |
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| Rok vydání: | 2006 |
| Předmět: |
medicine.disease_cause
Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Bacterial Proteins Microscopy Electron Transmission Biosynthesis Polysaccharides medicine Escherichia coli Escherichia coli K12 Ecology Strain (chemistry) biology Escherichia coli Proteins Biofilm Physiology and Biotechnology biology.organism_classification Enterobacteriaceae Transformation (genetics) chemistry Biofilms Fimbriae Bacterial bacteria Genetic Engineering Gene Deletion Bacteria Food Science Biotechnology Transformation efficiency |
| Zdroj: | Applied and Environmental Microbiology. 72:3336-3342 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.72.5.3336-3342.2006 |
| Popis: | Bacteria form biofilms by adhering to biotic or abiotic surfaces. This phenomenon causes several problems, including a reduction in the transport of mass and heat, an increase in resistance to antibiotics, and a shortening of the lifetimes of modules in bioindustrial fermentors. To overcome these difficulties, we created a biofilm production-deficient Escherichia coli strain, BD123, by deleting genes involved in curli biosynthesis and assembly, Δ( csgG-csgC ); colanic acid biosynthesis and assembly, Δ( wcaL-wza ); and type I pilus biosynthesis, Δ( fimB-fimH ). E. coli BD123 remained mostly in the form of planktonic cells under the conditions tested and became more sensitive to the antibiotics streptomycin and rifampin than the wild-type E. coli MG1655: the growth of BD123 was inhibited by one-fourth of the concentrations needed to inhibit MG1655. In addition, the transformation efficiency of BD123 was about 20 times higher than that of MG1655, and the production and secretion of recombinant proteins were ∼16% and ∼25% greater, respectively, with BD123 than with MG1655. These results indicate that the newly created biofilm production-deficient strain of E. coli displays several key properties that substantially enhance its utility in the biotechnology arena. |
| Databáze: | OpenAIRE |
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