An adult tissue-specific stem cell in its niche: A gene profiling analysis of in vivo quiescent and activated muscle satellite cells
| Autor: | Didier Montarras, Stéphanie François, Ana Cumano, Bertrand Czarny, Giorgia Pallafacchina, Béatrice Regnault, Vincent Dive, Margaret Buckingham |
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| Přispěvatelé: | Génétique Moléculaire du Développement, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Génopole, Institut Pasteur [Paris] (IP), Département d'Ingénierie et Etudes des Protéines, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Développement des Lymphocytes, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris], Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
MESH: Signal Transduction
Cell Fluorescent Antibody Technique MESH: Flow Cytometry Polymerase Chain Reaction Extracellular matrix Transcriptome Mice 0302 clinical medicine MESH: Eye Proteins MESH: Gene Expression Regulation Developmental MESH: Animals MESH: Fluorescent Antibody Technique Cells Cultured Oligonucleotide Array Sequence Analysis Medicine(all) 0303 health sciences Stem Cells MESH: Satellite Cells Skeletal Muscle Gene Expression Regulation Developmental General Medicine Flow Cytometry Cell biology medicine.anatomical_structure [SDV.IMM]Life Sciences [q-bio]/Immunology Stem cell Cell activation Signal Transduction MESH: Cells Cultured Satellite Cells Skeletal Muscle MESH: Mice Transgenic Blotting Western Mice Transgenic MESH: Stem Cells Biology 03 medical and health sciences MESH: Gene Expression Profiling MESH: Cell Proliferation medicine Animals MESH: Blotting Western Eye Proteins Cell adhesion MESH: Mice Cell Proliferation 030304 developmental biology Gene Expression Profiling Skeletal muscle MESH: Polymerase Chain Reaction Cell Biology Cell culture MESH: Oligonucleotide Array Sequence Analysis 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cell Research Stem Cell Research; Vol 4 Stem Cell Research, 2010, 4 (2), pp.77-91. ⟨10.1016/j.scr.2009.10.003⟩ Stem Cell Research, Elsevier, 2010, 4 (2), pp.77-91. ⟨10.1016/j.scr.2009.10.003⟩ |
| ISSN: | 1873-5061 1876-7753 |
| DOI: | 10.1016/j.scr.2009.10.003 |
| Popis: | International audience; The satellite cell of skeletal muscle provides a paradigm for quiescent and activated tissue stem cell states. We have carried out transcriptome analyses on satellite cells purified by flow cytometry from Pax3(GFP/+) mice. We compared samples from adult skeletal muscles where satellite cells are mainly quiescent, with samples from growing muscles or regenerating (mdx) muscles, where they are activated. Analysis of regulation that is shared by both activated states avoids other effects due to immature or pathological conditions. This in vivo profile differs from that of previously analyzed satellite cells activated after cell culture. It reveals how the satellite cell protects itself from damage and maintains quiescence, while being primed for activation on receipt of the appropriate signal. This is illustrated by manipulation of the corepressor Dach1, and by the demonstration that quiescent satellite cells are better protected from oxidative stress than those from mdx or 1-week-old muscles. The quiescent versus in vivo activated comparison also gives new insights into how the satellite cell controls its niche on the muscle fiber through cell adhesion and matrix remodeling. The latter also potentiates growth factor activity through proteoglycan modification. Dismantling the extracellular matrix is important for satellite cell activation when the expression of proteinases is up-regulated, whereas transcripts for their inhibitors are high in quiescent cells. In keeping with this, we demonstrate that metalloproteinase function is required for efficient regeneration in vivo. |
| Databáze: | OpenAIRE |
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