A strategy for practical authentication of medicinal plants in traditional Chinese medicine prescription, paeony root in ShaoYao-GanCao decoction as a case study
| Autor: | Yun Li, Hao-Jv Li, Hua Qu, De-An Guo, Wenlong Wei, Jiayuan Li, Jianqing Zhang, Mengzhen Cheng, Zhenwei Li, Shuai Yao, Changliang Yao |
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| Rok vydání: | 2021 |
| Předmět: |
Molecular Conformation
Filtration and Separation Decoction 02 engineering and technology Traditional Chinese medicine Paeonia 01 natural sciences Plant Roots Analytical Chemistry Chemometrics chemistry.chemical_compound Chemical marker Monitoring methods Medicine Chinese Traditional Medicinal plants Chromatography High Pressure Liquid Mathematics Flavonoids Plants Medicinal Traditional medicine 010401 analytical chemistry Saponins 021001 nanoscience & nanotechnology Paeoniflorin Quality enhancement 0104 chemical sciences chemistry Monoterpenes 0210 nano-technology Drugs Chinese Herbal |
| Zdroj: | Journal of separation scienceREFERENCES. 44(12) |
| ISSN: | 1615-9314 |
| Popis: | Authentication of Chinese medicine materials in prescriptions is extremely difficult due to the complicated chemical matrix. A strategy integrating in-depth profiling, chemical marker selection, and selected detection was established and exemplarily applied to authenticate paeony root in ShaoYao-GanCao decoction. Firstly, an ultra-performance LC/linear trap quadrupole-Orbitrap method was developed to probe the chemical compositions of the decoction. Secondly, 20 batches of decoctions prepared from white paeony root and red paeony root were compared by a metabolomics method, and multi-step chemometrics analysis distinguished the chemical markers. Thirdly, an ultra-performance LC/QDa-selected ion monitoring method was developed to authenticate the paeony root in decoctions. As a result, 161 compounds were characterized, including 84 triterpene saponins, 42 flavonoids and 10 monoterpenes. Four chemical markers and paeoniflorin were successfully screened out as chemical markers for white paeony root. The selected ion monitoring method easily differentiated authentic decoction (prepared from white paeony root) from fraud decoction (prepared from red paeony root) by monitoring the above five chemical markers. In conclusion, the strategy was proved effective in authentication of paeony root in ShaoYao-GanCao decoction, and it can also be applied to authenticate other Chinese medicine materials in prescriptions, which will greatly avail the quality enhancement of prescriptions. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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