Water-compatible imprinted pills for sensitive determination of cannabinoids in urine and oral fluid
| Autor: | J.M. López-Vilariño, Elena Lendoiro, Ana de Castro, Cristian Jiménez-Morigosa, Angelines Cruz, Ferdia Bates, M. Concepción Cela-Pérez, Manuel López-Rivadulla, M. Victoria González-Rodríguez |
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| Rok vydání: | 2015 |
| Předmět: |
Formic acid
Polymers Ethylene glycol dimethacrylate 02 engineering and technology Urine 01 natural sciences Biochemistry Chemistry Techniques Analytical Analytical Chemistry chemistry.chemical_compound Liquid chromatography–mass spectrometry Tandem Mass Spectrometry Humans Solid phase extraction Dronabinol Saliva Chromatography Elution Cannabinoids 010401 analytical chemistry Organic Chemistry Extraction (chemistry) Solid Phase Extraction Molecularly imprinted polymer Water General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences Body Fluids chemistry Cannabinol Solvents Methacrylates 0210 nano-technology Chromatography Liquid |
| Zdroj: | Journal of chromatography. A. 1429 |
| ISSN: | 1873-3778 |
| Popis: | A novel molecularly imprinted solid phase extraction (MISPE) methodology followed by liquid chromatography tandem mass spectrometry (LC-MS/MS) has been developed using cylindrical shaped molecularly imprinted pills for detection of Δ(9)-tetrahydrocannabinol (THC), 11-nor-Δ(9)-tetrahydrocannabinol carboxylic acid (THC-COOH), cannabinol (CBN) and cannabidiol (CBD) in urine and oral fluid (OF). The composition of the molecular imprinted polymer (MIP) was optimized based on the screening results of a non-imprinted polymer library (NIP-library). Thus, acrylamide as functional monomer and ethylene glycol dimethacrylate as cross-linker were selected for the preparation of the MIP, using catechin as a mimic template. MISPE pills were incubated with 0.5 mL urine or OF sample for adsorption of analytes. For desorption, the pills were transferred to a vial with 2 mL of methanol:acetic acid (4:1) and sonicated for 15 min. The elution solvent was evaporated and reconstituted in methanol:formic acid (0.1%) 50:50 to inject in LC-MS/MS. The developed method was linear over the range from 1 to 500 ng mL(-1) in urine and from 0.75 to 500 ng mL(-1) in OF for all four analytes. Intra- and inter-day imprecision were |
| Databáze: | OpenAIRE |
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