A Continuous Cell Separation and Collection Approach on a Microfilter and Negative Dielectrophoresis Combined Chip
| Autor: | Danfen Yin, Jun Yang, Xiaoling Zhang, Ning Hu, Qiong Wang, Jinan Deng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Algal cells
Materials science lcsh:Mechanical engineering and machinery 02 engineering and technology 01 natural sciences Negative dielectrophoresis Article law.invention law Cell separation lcsh:TJ1-1570 Electrical and Electronic Engineering microfilter Throughput (business) Filtration dielectrophoresis business.industry Mechanical Engineering 010401 analytical chemistry micropost array railing Dielectrophoresis 021001 nanoscience & nanotechnology Chip 0104 chemical sciences Control and Systems Engineering Electrode particle/cell separation Optoelectronics 0210 nano-technology business |
| Zdroj: | Micromachines, Vol 11, Iss 1037, p 1037 (2020) Micromachines Volume 11 Issue 12 |
| Popis: | Cell separation plays an important role in the fields of analytical chemistry and biomedicine. To solve the blockage problem and improve the separation throughput in the traditional microstructure filtration-based separation approach, a continuous cell separation and collection approach via micropost array railing on a microfilter and negative dielectrophoresis combined chip is proposed. By tilting the micropost array at a certain angle, microparticles or cells enter the collection area under micropost array railing. The effects of the inclination angle of the micropost array and the electrode distance on the microparticle collection efficiency were investigated. Based on the optimized microfluidic chip structure, 37- and 16.3-&mu m particles were collected with 85% and 89% efficiencies, respectively. Additionally, algal cells were separated and collected by using the optimized microchip. The chip also had good separation and collection effects on biological samples, which effectively solved the blockage problem and improved the separation throughput, laying a foundation for subsequent microstructure filtration separation-based research and application. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|