Irc20 Regulates the Yeast Endogenous 2-μm Plasmid Levels by Controlling Flp1
| Autor: | Ahmed H. Hassan, Jisha Chalissery, Deena Jalal |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Flp1 Regulator homologous recombination Biochemistry Genetics and Molecular Biology (miscellaneous) Biochemistry ubiquitin ligase 03 medical and health sciences 0302 clinical medicine Plasmid Ubiquitin Recombinase Molecular Biosciences Molecular Biology lcsh:QH301-705.5 biology Chemistry DNA replication Irc20 Brief Research Report Yeast Cell biology Ubiquitin ligase 030104 developmental biology lcsh:Biology (General) SUMO 030220 oncology & carcinogenesis biology.protein 2-μm plasmid Homologous recombination |
| Zdroj: | Frontiers in Molecular Biosciences, Vol 7 (2020) Frontiers in Molecular Biosciences |
| DOI: | 10.3389/fmolb.2020.00221/full |
| Popis: | The endogenous yeast 2-μm plasmid while innocuous to the host, needs to be properly regulated to avoid a toxic increase in copy number. The plasmid copy number control system is under the control of the plasmid encoded recombinase, Flp1. In case of a drop in 2-μm plasmid levels due to rare plasmid mis-segregation events, the Flp1 recombinase together with the cell’s homologous recombination machinery, produce multiple copies of the 2-μm plasmid that are spooled during DNA replication. The 2-μm plasmid copy number is tightly regulated by controlled expression of Flp1 as well as its ubiquitin and SUMO modification. Here, we identify a novel regulator of the 2-μm plasmid, the ATPase, ubiquitin ligase, Irc20. Irc20 was initially identified as a homologous recombination regulator, and here we uncover a new role for Irc20 in maintaining the 2-μm plasmid copy number and segregation through regulating Flp1 protein levels in the cell. |
| Databáze: | OpenAIRE |
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