DDT-RELATED PROTEIN4-IMITATION SWITCH alters nucleosome distribution to relieve transcriptional silencing in Arabidopsis
| Autor: | Zhang, Qiyan, Wang, Zejia, Lu, Xinyue, Yan, Huiru, Zhang, Huawei, He, Hang, Bischof, Sylvain, Harris, C Jake, Liu, Qikun |
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| Přispěvatelé: | Zhang, Qiyan [0000-0002-2446-0082], Wang, Zejia [0000-0002-0032-2662], Lu, Xinyue [0000-0002-3362-0092], Yan, Huiru [0009-0009-4758-1205], Zhang, Huawei [0000-0002-8893-1429], He, Hang [0000-0003-3165-283X], Bischof, Sylvain [0000-0003-2910-5132], Harris, C Jake [0000-0001-5120-0377], Liu, Qikun [0000-0003-2489-2907], Apollo - University of Cambridge Repository, University of Zurich |
| Rok vydání: | 2023 |
| Předmět: |
1.1 Normal biological development and functioning
Human Genome Cell Biology Plant Science 580 Plants (Botany) 3108 Plant Biology 3105 Genetics 10126 Department of Plant and Microbial Biology FOS: Biological sciences Genetics 1 Underpinning research Generic health relevance 10211 Zurich-Basel Plant Science Center 31 Biological Sciences |
| Popis: | DNA methylation is a conserved epigenetic modification that is typically associated with silencing of transposable elements and promoter methylated genes. However, some DNA-methylated loci are protected from silencing, allowing transcriptional flexibility in response to environmental and developmental cues. Through a genetic screen in Arabidopsis (Arabidopsis thaliana), we uncovered an antagonistic relationship between the MICRORCHIDIA (MORC) protein and the IMITATION SWITCH (ISWI) complex in regulating the DNA-methylated SUPPRESSOR OF DRM1 DRM2 CMT3 (SDC) reporter. We demonstrate that components of the plant-specific ISWI complex, including CHROMATIN REMODELING PROTEIN11 (CHR11), CHR17, DDT-RELATED PROTEIN4 (DDR4), and DDR5, function to partially de-repress silenced genes and transposable elements (TEs), through their function in regulating nucleosome distribution. This action also requires the known transcriptional activator DNAJ proteins, providing a mechanistic link between nucleosome remodeling and transcriptional activation. Genome-wide studies revealed that DDR4 causes changes in nucleosome distribution at numerous loci, a subset of which is associated with changes in DNA methylation and/or transcription. Our work reveals a mechanism for balancing transcriptional flexibility and faithful silencing of DNA-methylated loci. As both ISWI and MORC family genes are widely distributed across plant and animal species, our findings may represent a conserved eukaryotic mechanism for fine-tuning gene expression under epigenetic regulation. |
| Databáze: | OpenAIRE |
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