Isolation, Purification, and Characterization of Leptophages
| Autor: | Olivier Schiettekatte, Pascale Bourhy |
|---|---|
| Přispěvatelé: | Biologie des Spirochètes / Biology of Spirochetes, Institut Pasteur [Paris], École Doctorale Bio Sorbonne Paris Cité [Paris] (ED BioSPC), Université Sorbonne Paris Cité (USPC)-Université de Paris (UP), This work was part of the Ph.D. thesis of O.S. who received financial support from 'Université Paris Diderot' and 'Sorbonne Paris Cité ́.', We thank Soizick Lucas-Staat and Nicolas Dufour for technical assistance during leptophage extractions, Olivier Gorgette for technical assistance during the TEM observations, and Robert A. Gaultney for proofreading the manuscript., Institut Pasteur [Paris] (IP), École Doctorale Bio Sorbonne Paris Cité [Paris] (ED562 - BioSPC), Université Sorbonne Paris Cité (USPC)-Université Paris Cité (UPCité) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
030231 tropical medicine 030106 microbiology Mitomycin C Biology Isolation (microbiology) Genome TEM characterization 3. Good health Microbiology Polyethylene glycol precipitation Cesium chloride purification 03 medical and health sciences 0302 clinical medicine Leptophages [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Genus One-step growth kinetics Prophage |
| Zdroj: | Leptospira spp. Leptospira spp., 2134, Humana, pp.67-75, 2020, Methods in Molecular Biology, 978-1-0716-0459-5. ⟨10.1007/978-1-0716-0459-5_7⟩ Methods in Molecular Biology ISBN: 9781071604588 |
| DOI: | 10.1007/978-1-0716-0459-5_7⟩ |
| Popis: | Cite this protocol as:Schiettekatte O., Bourhy P. (2020) Isolation, Purification, and Characterization of Leptophages. In: Koizumi N., Picardeau M. (eds) Leptospira spp.. Methods in Molecular Biology, vol 2134. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0459-5_7; International audience; To date, only three bacteriophages of leptospires-leptophages-are known. Nonetheless, numerous prophages have been found in the genus, especially in the genomes of pathogenic species. Thus, some laboratories attempt to isolate leptophage particles from environmental samples or following mitomycin C induction of bacterial cultures. Here, we propose multiple procedures to isolate, purify, and characterize bacteriophages, based on protocols used for LE3 and LE4 characterization. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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