A novel plant ferredoxin-like protein and the regulator Hor are quorum-sensing targets in the plant pathogen Erwinia carotovora
| Autor: | Heidi Harjunpää, E. Tapio Palva, Günter Brader, Solveig Sjöblom |
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| Rok vydání: | 2008 |
| Předmět: |
DNA
Bacterial DNA Plant Physiology Molecular Sequence Data Arabidopsis Virulence Pectobacterium carotovorum Biology Erwinia Genes Plant Models Biological Bacterial genetics Microbiology 03 medical and health sciences Bacterial Proteins Amino Acid Sequence 030304 developmental biology Regulator gene DNA Primers Plant Diseases Plant Proteins Solanum tuberosum 0303 health sciences Base Sequence Sequence Homology Amino Acid 030306 microbiology Genetic transfer food and beverages Quorum Sensing General Medicine biology.organism_classification Plants Genetically Modified Quorum sensing Mutagenesis Insertional Oxidative Stress Genes Bacterial Host-Pathogen Interactions Trans-Activators Transposon mutagenesis Agronomy and Crop Science |
| Zdroj: | Molecular plant-microbe interactions : MPMI. 21(7) |
| ISSN: | 0894-0282 |
| Popis: | Quorum sensing (QS), a population-density-sensing mechanism, controls the production of the main virulence determinants, the plant cell-wall-degrading enzymes (PCWDEs) of the soft-rot phytopathogen Erwinia carotovora subsp. carotovora. In this study, we used random transposon mutagenesis with a gusA reporter construct to identify two new QS-controlled genes encoding the regulator Hor and a plant ferredoxin-like protein, FerE. The QS control of the identified genes was executed by the QS regulators ExpR1 and ExpR2 and mediated by the global repressor RsmA. Hor was shown to contribute to bacterial virulence at least partly through its control of PCWDE production. Our results showed that FerE contributes to oxidative stress tolerance and in planta fitness of the bacteria and suggest that QS could be central to control of oxidative stress tolerance. The presence of the FerE protein appears to be rather unique in heterotrophic bacteria and suggests an acquisition of the corresponding gene from plant host by horizontal gene transfer. |
| Databáze: | OpenAIRE |
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