Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids
| Autor: | Eduardo Rodríguez-Noriega, María Dolores Alcántar-Curiel, José Alejandro Di Conza, Rayo Morfin-Otero, José Eduardo Toledano-Tableros, Gabriel Osvaldo Gutkind, Catalina Gayosso-Vázquez, Silvia Giono-Cerezo, Ma Dolores Jarillo-Quijada, José Luis Fernández-Vázquez, José Ignacio Santos-Preciado |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Klebsiella pneumoniae lcsh:QR1-502 Aztreonam Microbiology lcsh:Microbiology law.invention 03 medical and health sciences chemistry.chemical_compound Antibiotic resistance Plasmid law Pulsed-field gel electrophoresis medicine blaNDM–1 Mexico Polymerase chain reaction 030304 developmental biology 0303 health sciences biology 030306 microbiology biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification IncF-like plasmids chemistry Colistin Multilocus sequence typing carbapenems medicine.drug MLST |
| Zdroj: | Frontiers in Microbiology, Vol 12 (2021) |
| Popis: | Nosocomial infections caused by multidrug-resistant (MDR) Klebsiella pneumoniae are a major health problem worldwide. The aim of this study was to describe NDM-1-producing K. pneumoniae strains causing bacteremia in a tertiary referral hospital in Mexico. MDR K. pneumoniae isolates were screened by polymerase chain reaction for the presence of resistance genes. In resistant isolates, plasmids were identified and conjugation assays were performed. Clonal diversity and the sequence types were determined by pulsed-field gel electrophoresis and multilocus sequence typing. A total of 80 K. pneumoniae isolates were collected from patients with bacteremia over a 1-year period. These isolates showed a level of resistance of 59% (47/80) to aztreonam, 56–60% (45–48/80) to cephalosporins, 54% (43/80) to colistin and 12.5% (10/80) to carbapenems. The carbapenem resistant isolates were blaNDM–1 carriers and negative for blaKPC, blaNDM, blaIMP, blaVIM and blaOXA–48–like carbapenemases genes. Conjugative plasmids IncFIIA and IncF group with sizes of 82–195 kbp were carriers of blaNDM–1, blaCTX–M–15, blaTEM–1, aac(6′)-Ib and/or aac(3′)-IIa. Clonal variability and nine different multilocus sequence types were detected (ST661, ST683, ST1395, ST2706, ST252, ST1198, ST690, ST1535, and ST3368) for the first time in the isolates carrying blaNDM–1 in Mexico. This study demonstrates that blaNDM–1 has remained within this hospital in recent years and suggests that it is currently the most prevalent carbapenemase among K. pneumoniae MDR strains causing bacteremia in Mexico. The horizontal transfer of blaNDM–1 gene through IncF-like plasmids among different clones demonstrates the dissemination pathway of antimicrobial resistance and underscore the need for strong and urgent joint measures to control the spread of NDM-1 carbapenemase in the hospital. |
| Databáze: | OpenAIRE |
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