Lack of genotoxicity of bitumen fumes in transgenic mouse lung
| Autor: | Gerard Keith, Stéphane Binet, C. Coulais, Marie Claire Bottin, David Moulin, Jean Claude Micillino, Bertrand H. Rihn |
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| Přispěvatelé: | Département Polluants et Santé, Institut national de recherche et de sécurité (Vandoeuvre lès Nancy) (INRS ( Vandoeuvre lès Nancy)) |
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
lacI
Mouse [SDV]Life Sciences [q-bio] Mutant Mice Transgenic 010501 environmental sciences Toxicology medicine.disease_cause 01 natural sciences Transgenic Adduct Mice 03 medical and health sciences chemistry.chemical_compound Big Blue Tar (tobacco residue) Genes Reporter medicine otorhinolaryngologic diseases Animals Lung Carcinogen 030304 developmental biology 0105 earth and related environmental sciences Risk assessment Aerosols Genetics 0303 health sciences Mutation Spectra Mutagenicity Tests Mutagenesis DNA adducts DNA Molecular biology Hydrocarbons 3. Good health Mice Inbred C57BL Fixation time Lac Operon chemistry Inhalation Mutation Bitumen Chromatography Thin Layer Gases Genotoxicity Mutagens cII |
| Zdroj: | Toxicology Toxicology, Elsevier, 2002, 170 (1-2), pp.11-20. ⟨10.1016/S0300-483X(01)00507-8⟩ |
| ISSN: | 0300-483X |
| Popis: | International audience; During hot application of bitumen containing materials, e.g. in hot paving or roofing, fumes are emitted that contain polycyclic aromatic compounds. Previous studies with rodents exposed to bitumen and coal-tar fume condensates showed formation of DNA adducts. In order to clarify the genotoxicity of bitumen fumes, we designed a study by using mice carrying a reporter gene for mutagenesis analysis and exposed by nose-only to a constant and reproducible aerosol of bitumen fumes. We analyzed the genotoxic activity of inhaled bitumen fumes generated under those controlled conditions through the induction of mutation and DNA adducts in Big Blue mice. Mice were exposed to bitumen fumes (100 mg/m(3) total particulate matter) 6 h per day during 5 days by nose-only in an inhalation chamber designed in our laboratory. Following a 30-day fixation period, the experiment was terminated and lung DNA was extracted for mutant frequency and adduct determinations. The mutant frequency was determined using the cII and the lacI mutant analysis systems. In, addition, 61 and 54 mutants were sequenced in control and exposed groups, respectively. The study did not show any mutation or adduct induction in the exposed group compared to the control group: cII mutant frequencies were 11.0+/-4.5x10(-5) and 11.0+/-4.8x10(-5) in control and exposed lungs, respectively. Identically, using the lacI mutation detection system, the mutant frequencies were 6.4+/-3.1x10(-5) and 5.8+/-2.0x10(-5). The mutation spectra of both series were quite similar with regard to transition and transversion frequencies. The absence of genotoxicity in the group exposed to 100 mg/m(3) bitumen is discussed with regard to dosage of inhaled polycyclic aromatic compounds and species. |
| Databáze: | OpenAIRE |
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