Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi
| Autor: | Rosana Rodrigues, André Teixeira-Ferreira, André de Oliveira Carvalho, Valdirene Moreira Gomes, Gabriel Bonan Taveira, Marciele Souza da Silva, Lídia da Silva Pereira, Rodrigo da Silva Gebara, Jonas Perales, Érica O. Mello, Layrana de Azevedo Dos Santos |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Fusarium Pore Forming Cytotoxic Proteins Antimicrobial peptides Biophysics Plant Biology Fungus 01 natural sciences Biochemistry 03 medical and health sciences Fusarium oxysporum Molecular Biology Defensin Plant peptides Research Articles Microbial Viability biology Plant Extracts food and beverages Cell Biology lipid transfer protein biology.organism_classification Antimicrobial Therapeutics & Molecular Medicine Capsicum chinense Fungicides Industrial 030104 developmental biology Fruit Capsicum Fusarium solani 010606 plant biology & botany Biotechnology mechanism of action |
| Zdroj: | Bioscience Reports |
| ISSN: | 1573-4935 0144-8463 |
| Popis: | In recent years, the antimicrobial activity of peptides isolated from a wide variety of organs from plant species has been reported. However, a few studies have investigated the potential of antimicrobial peptides (AMPs) found in fruits, especially Capsicum chinense (pepper). The present study aimed to purify and characterize peptides from Capsicum chinense fruits and evaluate their inhibitory activities against different phytopathogenic fungi and also analyze the possible mechanisms of action involved in microbial inhibition. After fruit protein extraction and high-performance liquid chromatography (HPLC), different fractions were obtained, named F1 to F10. Peptides in the F4 and F5 fractions were sequenced and revealed similarity with the plant antimicrobial peptides like non-specific lipid transfer proteins and defensin-like peptide. The F4 and F5 fractions presented strong antimicrobial activity against the fungus Fusarium solani and Fusarium oxysporum, causing toxic effects on these fungi, leading to membrane permeabilization, endogenous reactive oxygen species increase, activation of metacaspase and loss of mitochondrial function. |
| Databáze: | OpenAIRE |
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