PCR amplification of SRY-related gene sequences reveals evolutionary conservation of the SRY-box motif
| Autor: | A M Coriat, J L Harry, Paul T. Sharpe, Dafe Uwanogho, Ulrike Müller |
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| Předmět: |
Molecular Sequence Data
Biology Y chromosome Polymerase Chain Reaction Homology (biology) Conserved sequence Genetics Animals Humans Amino Acid Sequence Gene Genetics (clinical) Regulator gene Alligators and Crocodiles Base Sequence Sequence Homology Amino Acid Nuclear Proteins Lizards DNA Biological Evolution Sex-Determining Region Y Protein DNA-Binding Proteins Open reading frame genomic DNA Testis determining factor Drosophila Chickens Transcription Factors |
| Zdroj: | Europe PubMed Central |
| Popis: | SRY (sex-determining region of the Y chromosome) has recently been identified as a key regulatory gene in mammalian sex determination. The open reading frame of this gene contains an 80-amino-acid motif, the SRY-box, which shares a high degree of homology with a DNA-binding domain found in the high-mobility-group (HMG) proteins HMG1 and HMG2. The SRY box motif is highly conserved in several sequence-specific DNA-binding proteins that are known to act as transcription factors. Here we describe the use of degenerate PCR primers to identify SRY-related sequences containing the SRY-box motif from the genomic DNA of a variety of species. The results of this study suggest that in a diverse array of species SRY-related genes may serve as transcription factors that regulate a variety of developmental pathways, including sex determination. |
| Databáze: | OpenAIRE |
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