Human adipose-derived mesenchymal stromal cells increase endogenous neurogenesis in the rat subventricular zone acutely after 6-hydroxydopamine lesioning
| Autor: | Andreas Kurtz, Manfred Roch, Jürgen Berg, Jennifer Altschüler, Anne Schwerk, Christine Winter, Barbara Steiner, Manfred Gossen |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Cancer Research Pathology medicine.medical_specialty Neurogenesis Immunology Cell- and Tissue-Based Therapy Subventricular zone Substantia nigra S100 Calcium Binding Protein beta Subunit Biology Mesenchymal Stem Cell Transplantation Young Adult Neurotrophic factors Lateral Ventricles medicine Animals Humans Immunology and Allergy Obesity Rats Wistar Oxidopamine Cells Cultured Genetics (clinical) Cell Proliferation Transplantation Hydroxydopamine Brain-Derived Neurotrophic Factor Mesenchymal stem cell Mesenchymal Stem Cells Parkinson Disease Cell Biology Olfactory Bulb Rats Substantia Nigra Disease Models Animal medicine.anatomical_structure Adipose Tissue nervous system Oncology Female Adult stem cell |
| Zdroj: | Cytotherapy. 17:199-214 |
| ISSN: | 1465-3249 |
| DOI: | 10.1016/j.jcyt.2014.09.005 |
| Popis: | Background aims In Parkinson's disease (PD), neurogenesis in the subventricular zone (SVZ)–olfactory bulb (OB) axis is affected as the result of the lack of dopaminergic innervations reaching the SVZ. This aberrant network has been related to the hyposmia of PD patients, which is an early diagnostic marker of the disease. Consequently, much interest arose in finding mechanisms to modulate the SVZ-OB axis. Direct modulation of this axis could be achieved by transplantation of mesenchymal stromal cells (MSC), as it has been shown in rat and mouse PD models. However, the neurogenic effect of MSC in PD was thus far only analyzed weeks after transplantation, and little is known about effects immediately after transplantation. Methods We assessed the acute neuroprotective and neurogenic effects of adipose-derived MSC transplanted into the rat substantia nigra in the 6-hydroxydopamine model of PD. Results Three days after transplantation, subventricular neurogenesis was significantly increased in MSC-transplanted versus non-transplanted animals. Most MSC were found in the region of the substantia nigra and the surrounding arachnoid mater, expressing S100β and brain-derived neurotrophic factor, whereas some MSC showed an endothelial phenotype and localized around blood vessels. Conclusions The acute neurogenic effects and neurotrophic factor expression of MSC could help to restore the SVZ-OB axis in PD. |
| Databáze: | OpenAIRE |
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