Structure of SARS-CoV-2 ORF8, a rapidly evolving immune evasion protein
| Autor: | Flower, Thomas G., Buffalo, Cosmo Z., Hooy, Richard M., Allaire, Marc, Ren, Xuefeng, Hurley, James H. |
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| Rok vydání: | 2021 |
| Předmět: |
2019-20 coronavirus outbreak
Coronavirus disease 2019 (COVID-19) Evolution Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Dimer viruses Article Evolution Molecular Vaccine Related chemistry.chemical_compound Viral Proteins Immune system Biodefense 2.1 Biological and endogenous factors Aetiology skin and connective tissue diseases Lung Sequence (medicine) Immune Evasion X-ray crystallography Multidisciplinary Molecular Structure Chemistry SARS-CoV-2 Prevention fungi virus diseases Molecular COVID-19 Pneumonia biochemical phenomena metabolism and nutrition Evasion (ethics) Cell biology body regions Emerging Infectious Diseases Infectious Diseases Good Health and Well Being Pneumonia & Influenza |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America, vol 118, iss 2 Proceedings of the National Academy of Sciences bioRxiv article-version (status) pre article-version (number) 1 |
| Popis: | The molecular basis for the severity and rapid spread of the COVID-19 disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is largely unknown. ORF8 is a rapidly evolving accessory protein that has been proposed to interfere with immune responses. The crystal structure of SARS-CoV-2 ORF8 was determined at 2.04-Å resolution by X-ray crystallography. The structure reveals a ∼60-residue core similar to SARS-CoV-2 ORF7a, with the addition of two dimerization interfaces unique to SARS-CoV-2 ORF8. A covalent disulfide-linked dimer is formed through an N-terminal sequence specific to SARS-CoV-2, while a separate noncovalent interface is formed by another SARS-CoV-2-specific sequence, 73YIDI76 Together, the presence of these interfaces shows how SARS-CoV-2 ORF8 can form unique large-scale assemblies not possible for SARS-CoV, potentially mediating unique immune suppression and evasion activities. |
| Databáze: | OpenAIRE |
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