Evaluation of cross-reactivity to Taenia hydatigena and Echinococcus granulosus in the enzyme-linked immunoelectrotransfer blot assay for the diagnosis of porcine cysticercosis

Autor: Lucho Gomez-Puerta, Pierre Dorny, Maria T. Lopez-Urbina, Yesenia Castillo, Ana Vargas-Calla, Seth E. O’Neal, Hector H. Garcia, Armando E. Gonzalez
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
glycoprotein
pig
Taenia hydatigena
parasitology
Swine
Porcine
purl.org/pe-repo/ocde/ford#3.03.07 [https]
Serology
lentil lectin glycoprotein enzyme linked immunoelectrotransfer blot assay
immunology
Epitopes
protein GP50
0302 clinical medicine
Taenia solium
Taeniasis
animal
cross reaction
Echinococcus granulosus
2. Zero hunger
Swine Diseases
epitope
biology
Cysticercosis
Helminth Proteins
Echinococcosis
enzyme immunoassay
3. Good health
unclassified drug
medicine.drug_formulation_ingredient
Infectious Diseases
Cross-reaction
helminth protein
Specificity
Enzyme-linked immunoelectrotransfer blot
cyst (resting stage)
immunoblotting
GP50
parasite antigen
taeniasis
Immunoblotting
030231 tropical medicine
animal experiment
Short Report
Cross Reactions
Article
animal tissue
lcsh:Infectious and parasitic diseases
03 medical and health sciences
autopsy
evaluation study
parasitic diseases
medicine
Animals
controlled study
lcsh:RC109-216
procedures
swine disease
nonhuman
Taenia
biology.organism_classification
medicine.disease
Virology
030104 developmental biology
veterinary medicine
experimental infection
sensitivity and specificity
Antigens
Helminth
Parasitology
Zdroj: Parasites & Vectors, Vol 12, Iss 1, Pp 1-7 (2019)
Parasites & Vectors
ISSN: 1756-3305
DOI: 10.1186/s13071-018-3279-5
Popis: Background Taenia solium is an important zoonotic parasite that infects humans as definitive host (taeniasis) and pigs as intermediate host (cysticercosis). Serological diagnosis of porcine cysticercosis is limited to antigen detection using ELISA, which is known to cross-react with other Taenia species, and antibody detection using the lentil-lectin glycoprotein enzyme-linked immunoelectrotransfer blot (LLGP EITB), which has not been adequately evaluated for cross-reactivity to other parasites. Field studies suggest that the GP50 diagnostic band of the LLGP EITB may cross-react to Taenia hydatigena, a common non-zoonotic parasitic infection of pigs. The objective of this study was to evaluate the specificity of the LLGP EITB assay in pigs infected experimentally with T. hydatigena and Echinococcus granulosus. Results Twelve three-month-old seronegative were divided into two groups; six were each given an oral challenge with a single gravid proglottid of T. hydatigena and the other six were each given an oral challenge with 50 gravid proglottids of E. granulosus. Serum samples were collected biweekly until 14 weeks when all pigs underwent a detailed necropsy. Taenia hydatigena cysticerci were found in two of six pigs from the first group. Four T. hydatigena-exposed pigs were seropositive at the GP50-band only on EITB LLGP; two of these had cysts at necropsy while no seronegative pigs had cysts. One E. granulosus-exposed pig was positive to EITB LLGP, again with reactivity only to GP50; all six pigs had hepatic echinococcosis on necropsy. Conclusion These results provide definitive evidence that the GP50 diagnostic band in pigs cross-reacts with T. hydatigena. Evidence of cross-reaction with E. granulosus was not conclusive. Electronic supplementary material The online version of this article (10.1186/s13071-018-3279-5) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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