Cell line donor genotype and its influence on experimental phenotype: Toll-like receptor SNPs and potential variability in innate immunity
| Autor: | Wenxiang Luo, De-Ann M. Pillers, Steven J. Schrodi, Jessica DeValk, Sara Tokarz, Bikash R. Pattnaik |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genotype Endocrinology Diabetes and Metabolism Single-nucleotide polymorphism Biology Biochemistry Models Biological Polymorphism Single Nucleotide Cell Line 03 medical and health sciences Endocrinology Genetics SNP Humans Allele Molecular Biology Genotyping Toll-like receptor Innate immune system Toll-Like Receptors Phenotype Immunity Innate 030104 developmental biology Signal Transduction |
| Zdroj: | Molecular genetics and metabolism. 118(3) |
| ISSN: | 1096-7206 |
| Popis: | Cell lines are used to model a disease and provide valuable information regarding phenotype, mechanism, and response to novel therapies. Derived from individuals of diverse genetic backgrounds, the cell's genetic complement predicts the phenotype, and although some lines have been sequenced, little emphasis has been placed on genotyping. Toll-like receptors (TLRs) are essential in initiating the inflammatory cascade in response to infection. TLR single nucleotide polymorphism (SNP) alleles may predict an altered innate immune response: a SNP can affect TLR-dependent pathways and may alter experimental results. Thus, genotype variation may have far-reaching implications when using cell lines to model phenotypes. We recommend that cell lines be genotyped and cataloged in a fashion similar to that used for bacteria, with cumulative information being archived in an accessible central database to facilitate the understanding of SNP cell phenotypes reported in the literature. |
| Databáze: | OpenAIRE |
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