In vitro test for the evaluation of the efficacy of topical products for the control of Cochliomyia hominivorax larvae
| Autor: | Eduardo Gilberti Nascimento, Rafael Paranhos de Mendonça, Sabrina Nathália Louzada Nogueira, Maria Mariana Alves Andrade, Maria Fernanda da Silva, Daniel Paulino Júnior, Maria Cecília Soares, Welber Daniel Zanetti Lopes, Vando Edésio Soares, Ricardo de Andrade Furtado, Lorena Lopes Ferreira |
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| Rok vydání: | 2020 |
| Předmět: |
Larva
Veterinary medicine In vitro test biology 040301 veterinary sciences 030231 tropical medicine 04 agricultural and veterinary sciences medicine.disease_cause biology.organism_classification medicine.disease In vitro 0403 veterinary science 03 medical and health sciences 0302 clinical medicine Infectious Diseases In vivo Infestation medicine Animal Science and Zoology Parasitology In vivo experiment Myiasis Cochliomyia hominivorax Research Article |
| Zdroj: | Parasitology |
| ISSN: | 1469-8161 0031-1820 |
| DOI: | 10.1017/s0031182020000499 |
| Popis: | Cochliomyia hominivorax larvae cause myiasis in animals and humans. To register a commercial product to control this dipteran is necessary to experiment on animals. The in vitro test was standardized to evaluate the larvicidal efficacy of commercial topical products. Five formulations were analysed in vitro and in vivo. For the in vitro test, a colony was formed and three replicates (n = 200) of each larval stage (L1, L2 and L3) were treated. The viability of the larvae was evaluated after 5 and 30 min, and at 1, 2, 6, 12, 24, 48, 60 and 72 h post-treatment (HPT). For the in vivo test, 30 bovines divided into six groups were castrated to achieve natural infestation with C. hominivorax. Animals in the treated groups received the product. Myiasis and efficacy were evaluated 12, 24, 36, 48, 60 and 72 HPT. Four formulations tested in the in vitro test achieved 100% efficacy at 24 HPT. In the in vivo experiment only one achieved 100% efficacy at 24 HPT. However, all products achieved the maximum efficacy by the end of study. The in vitro test developed here could be adopted to evaluate the efficacy of topical products for the control of C. hominivorax larvae. |
| Databáze: | OpenAIRE |
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