Triterpenes and the Antimycobacterial Activity of Duroia macrophylla Huber (Rubiaceae)
| Autor: | Daniela Fernandes Ramos, Lillian Lucas Carrion, Daiane Martins, Andersson Barison, Cecilia Veronica Nunez, Kahlil Schwanka Salomé, Pedro Eduardo Almeida da Silva |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Phytochemistry
Magnetic Resonance Spectroscopy Nuclear Magnetic Resonance Spectroscopy lcsh:Medicine Rubiaceae Anti-bacterial Agents Antimycobacterial Terpene chemistry.chemical_compound Duroia Macrophylla Extract Drug Isolation Heteronuclear Multiple Bond Correlation Tannin Derivative biology Traditional medicine Microbial Sensitivity Test Hydrolysis General Medicine Reproducibility Anti-Bacterial Agents Chemistry Phytochemical Minimum Inhibitory Concentration Research Article Antiinfective Agent Article Subject medicine.drug_class Carbon Nuclear Magnetic Resonance Microbial Sensitivity Tests General Biochemistry Genetics and Molecular Biology Plant Extract Unclassified Drug Phenols Botany Ursolic Acid medicine Phenol Plant Leaf Oleanolic Acid Phenol Derivative Duroia Macrophylla Dichloromethane General Immunology and Microbiology Plant Extracts lcsh:R Reproducibility of Results Mycobacterium tuberculosis Nonhuman biology.organism_classification Triterpenes Antibacterial Activity Plant Leaves Metabolism chemistry Drug Effect Flavonoid Proton Nuclear Magnetic Resonance Reproducibility Of Results Methanol Triterpene Tannins Mycobacterium Tuberculosis |
| Zdroj: | BioMed Research International, Vol 2013 (2013) BioMed Research International Repositório Institucional da FURG (RI FURG) Universidade Federal do Rio Grande (FURG) instacron:FURG Repositório Institucional do INPA Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
| ISSN: | 2314-6133 |
| DOI: | 10.1155/2013/605831 |
| Popis: | Duroia macrophyllapopularly known as “cabeça-de-urubú,” “apuruí,” or “puruí-grande-da-mata” occurs in the Amazon Forest. Its leaves and branches were collected twice and extracted with dichloromethane and methanol. All extracts were subjected to phytochemical investigation and terpenes and flavonoids were found in all dichloromethane and methanol extracts, respectively. Methanol extracts from both branches (1st collection) and leaves (2nd collection) presented hydrolyzed tannins, yet alkaloids were only detected in the dichloromethane and methanol extracts from branches at the 2nd collection. Phenol compounds were found in both dichloromethane extracts’ collections. The action of every extract was assayed againstMycobacterium tuberculosis(RMPr, H37Rv, and INHr strains), showing that the dichloromethane extract from leaves (1st collection) has the major biological activity, with a MIC of 6.25 μg/mL for the INHr strain, 25.0 μg/mL for the RMPr strain, and ≤6.25 μg/mL for the H37Rv strain. The chromatographic fractioning of the dichloromethane extract from leaves (1st collection) yielded the isolation of two triterpenes: oleanolic and ursolic acids, which were identified by NMR analysis and reported for the first time in theDuroiagenus. |
| Databáze: | OpenAIRE |
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