A Preclinical Model of Malignant Peripheral Nerve Sheath Tumor-like Melanoma Is Characterized by Infiltrating Mast Cells
| Autor: | Jennifer Landsberg, Meri Rogava, Albert J. Becker, Michael Hölzel, Tobias Bald, Stefanie Riesenberg, Nicole Glodde, Thomas Tüting, Göran Jönsson |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research Pathology medicine.medical_specialty Cell Gene Expression Malignant peripheral nerve sheath tumor Biology Nerve Sheath Neoplasms Cohort Studies Mice 03 medical and health sciences Immune system Peripheral Nervous System Neoplasms Cell Line Tumor medicine Animals Humans Mast Cells Melanoma neoplasms Tumor microenvironment Gene signature Microarray Analysis medicine.disease Mast cell 030104 developmental biology medicine.anatomical_structure Oncology Nerve sheath neoplasm |
| Zdroj: | Cancer Research. 76:251-263 |
| ISSN: | 1538-7445 0008-5472 |
| DOI: | 10.1158/0008-5472.can-15-1090 |
| Popis: | Human melanomas exhibit considerable genetic, pathologic, and microenvironmental heterogeneity. Genetically engineered mice have successfully been used to model the genomic aberrations contributing to melanoma pathogenesis, but their ability to recapitulate the phenotypic variability of human disease and the complex interactions with the immune system have not been addressed. Here, we report the unexpected finding that immune cell–poor pigmented and immune cell–rich amelanotic melanomas developed simultaneously in Cdk4R24C-mutant mice upon melanocyte-specific conditional activation of oncogenic BrafV600E and a single application of the carcinogen 7,12-dimethylbenz(a)anthracene. Interestingly, amelanotic melanomas showed morphologic and molecular features of malignant peripheral nerve sheath tumors (MPNST). A bioinformatic cross-species comparison using a gene expression signature of MPNST-like mouse melanomas identified a subset of human melanomas with a similar histomorphology. Furthermore, this subset of human melanomas was found to be highly associated with a mast cell gene signature, and accordingly, mouse MPNST-like melanomas were also extensively infiltrated by mast cells and expressed mast cell chemoattractants similar to human counterparts. A transplantable mouse MPNST-like melanoma cell line recapitulated mast cell recruitment in syngeneic mice, demonstrating that this cell state can directly reconstitute the histomorphologic and microenvironmental features of primary MPNST-like melanomas. Our study emphasizes the importance of reciprocal, phenotype-dependent melanoma–immune cell interactions and highlights a critical role for mast cells in a subset of melanomas. Moreover, our BrafV600E-Cdk4R24C model represents an attractive system for the development of therapeutic approaches that can target the heterogeneous tumor microenvironment characteristic of human melanomas. Cancer Res; 76(2); 251–63. ©2015 AACR. |
| Databáze: | OpenAIRE |
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