Tissue-Specific Functions of fem-2/PP2c Phosphatase and fhod-1/formin During Caenorhabditis elegans Embryonic Morphogenesis
| Autor: | Ryan B. Smit, David Pruyne, Osama Refai, Paul E. Mains, SarahBeth Votra |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Embryo Nonmammalian formin Morphogenesis morphogenesis embryo Formins QH426-470 Investigations Animals Genetically Modified 03 medical and health sciences C . elegans epidermis Embryonic morphogenesis Myosin Genetics Phosphoprotein Phosphatases Animals Sarcomere organization Cytoskeleton Caenorhabditis elegans Caenorhabditis elegans Proteins Molecular Biology Rho-associated protein kinase Genetics (clinical) Actin biology Microfilament Proteins Gene Expression Regulation Developmental biology.organism_classification Cell biology Alternative Splicing 030104 developmental biology Phenotype Organ Specificity C. elegans |
| Zdroj: | G3: Genes|Genomes|Genetics G3: Genes, Genomes, Genetics, Vol 8, Iss 7, Pp 2277-2290 (2018) |
| ISSN: | 2160-1836 |
| Popis: | The cytoskeleton is the basic machinery that drives many morphogenetic events. Elongation of the C. elegans embryo from a spheroid into a long, thin larva initially results from actomyosin contractility, mainly in the lateral epidermal seam cells, while the corresponding dorsal and ventral epidermal cells play a more passive role. This is followed by a later elongation phase involving muscle contraction. Early elongation is mediated by parallel genetic pathways involving LET-502/Rho kinase and MEL-11/MYPT myosin phosphatase in one pathway and FEM-2/PP2c phosphatase and PAK-1/p21 activated kinase in another. While the LET-502/MEL-11 pathway appears to act primarily in the lateral epidermis, here we show that FEM-2 can mediate early elongation when expressed in the dorsal and ventral epidermis. We also investigated the early elongation function of FHOD-1, a member of the formin family of actin nucleators and bundlers. Previous work showed that FHOD-1 acts in the LET-502/MEL-11 branch of the early elongation pathway as well as in muscle for sarcomere organization. Consistent with this, we found that lateral epidermal cell-specific expression of FHOD-1 is sufficient for elongation, and FHOD-1 effects on elongation appear to be independent of its role in muscle. Also, we found that fhod-1 encodes long and short isoforms that differ in the presence of a predicted coiled-coil domain. Based on tissue-specific expression constructions and an isoform-specific CRISPR allele, the two FHOD-1 isoforms show partially specialized epidermal or muscle function. Although fhod-1 shows only impenetrant elongation phenotypes, we were unable to detect redundancy with other C. elegans formin genes. |
| Databáze: | OpenAIRE |
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