Multiple cryopreservation-warming cycles, coupled with blastocyst biopsy, negatively affect IVF outcomes
| Autor: | Ashley Aluko, Alan S. Penzias, Denis A. Vaughan, Michele R. Hacker, Anna M. Modest, Kim L. Thornton, Denny Sakkas |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult medicine.medical_specialty Biopsy Aneuploidy Single Embryo Transfer Fertilization in Vitro Cryopreservation Article Miscarriage 03 medical and health sciences 0302 clinical medicine Pregnancy Stress Physiological medicine Humans Blastocyst Birth Rate Preimplantation Diagnosis Genetic testing Retrospective Studies 030219 obstetrics & reproductive medicine medicine.diagnostic_test business.industry Obstetrics Pregnancy Outcome Obstetrics and Gynecology medicine.disease Embryo Transfer 030104 developmental biology medicine.anatomical_structure Reproductive Medicine Female business Live birth Developmental Biology Boston |
| Zdroj: | Reprod Biomed |
| ISSN: | 1472-6491 |
| Popis: | Research question Do multiple cryopreservation–warming cycles, coupled with blastocyst biopsy, negatively affect IVF outcomes? Design Patients undergoing IVF with homologous single embryo transfer, and who underwent trophectoderm biopsy for preimplantation genetic testing for aneuploidy (PGT-A) between 2013 and 2017, were divided into three groups based on degree of embryonic micromanipulation: once-biopsied, once-cryopreserved (group BC, n = 2603), once-biopsied, twice-cryopreserved (group CBC, n = 95) and twice-biopsied, twice-cryopreserved (group BCBC, n = 15). The primary outcome was live birth; secondary outcomes included positive serum pregnancy test, clinical pregnancy and miscarriage. Results Group CBC had a significantly lower chance of live birth (adjusted RR 0.57, 95% CI 0.41 to 0.79) and clinical pregnancy (adjusted RR 0.67, 95% CI 0.53 to 0.85) compared with group BC. Miscarriage rates were similar between groups BC and CBC (adjusted RR 1.3, 95% CI 0.64 to 2.7). Conclusions Multiple cryopreservation–warming cycles, coupled with blastocyst biopsy, negatively affect IVF outcomes. Although PGT-A is thought to improve reproductive outcomes on a per transfer basis, caution must be exercised in counselling patients on the possibility of diminishing returns owing to further embryonic micromanipulation after an embryo has been cryopreserved. |
| Databáze: | OpenAIRE |
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