Presence of Bacterial DNA in Thrombotic Material of Patients with Myocardial Infarction

Autor: Rita Moure, Pablo Piñón-Esteban, Xacobe Flores-Ríos, Lucía Núñez, R Calviño-Santos, Grecia M Marrón-Liñares, Guillermo Aldama-López, Jorge Salgado-Fernández, F Rebollal-Leal, Mar Tomás, R Pan-Lizcano, J M Vazquez-Rodriguez, Manuel Hermida-Prieto, Germán Bou, Nicolás Vázquez-González
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
DNA
Bacterial
Male
Acute coronary syndrome
Mycoplasma pneumoniae
030106 microbiology
lcsh:Medicine
Acute myocardial infarction
030204 cardiovascular system & hematology
medicine.disease_cause
Real-Time Polymerase Chain Reaction
Microbiology
law.invention
03 medical and health sciences
0302 clinical medicine
Percutaneous Coronary Intervention
law
medicine
Humans
Acute Coronary Syndrome
lcsh:Science
Porphyromonas gingivalis
Polymerase chain reaction
Periodontal Diseases
Multidisciplinary
biology
business.industry
lcsh:R
Aggregatibacter actinomycetemcomitans
Pulpitis
Treponema denticola
Thrombosis
Middle Aged
medicine.disease
biology.organism_classification
Bacterial DNA
stomatognathic diseases
Real-time polymerase chain reaction
ST Elevation Myocardial Infarction
lcsh:Q
Female
Fusobacterium nucleatum
Bacterial infection
business
Zdroj: RUC. Repositorio da Universidade da Coruña
Universitat Oberta de Catalunya (UOC)
Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
RUC: Repositorio da Universidade da Coruña
Universidade da Coruña (UDC)
Popis: Infectious agents have been suggested to be involved in etiopathogenesis of Acute Coronary Syndrome (ACS). However, the relationship between bacterial infection and acute myocardial infarction (AMI) has not yet been completely clarified. The objective of this study is to detect bacterial DNA in thrombotic material of patients with ACS with ST-segment elevation (STEMI) treated with Primary Percutaneous Coronary Intervention (PPCI). We studied 109 consecutive patients with STEMI, who underwent thrombus aspiration and arterial peripheral blood sampling. Testing for bacterial DNA was performed by probe-based real-time Polymerase Chain Reaction (PCR). 12 probes and primers were used for the detection of Aggregatibacteractinomycetemcomitans, Chlamydiapneumoniae, viridans group streptococci, Porphyromonasgingivalis, Fusobacteriumnucleatum, Tannarellaforsythia, Treponemadenticola, Helycobacterpylori, Mycoplasmapneumoniae, Staphylococusaureus, Prevotella intermedia and Streptococcusmutans. Thus, DNA of four species of bacteria was detected in 10 of the 109 patients studied. The most frequent species was viridans group streptococci (6 patients, 5.5%), followed by Staphylococusaureus (2 patients, 1.8%). Moreover, a patient had DNA of Porphyromonasgingivalis (0.9%); and another patient had DNA of Prevotella intermedia (0.9%). Bacterial DNA was not detected in peripheral blood of any of our patients. In conclusion, DNA of four species of endodontic and periodontal bacteria was detected in thrombotic material of 10 STEMI patients. Bacterial DNA was not detected in the peripheral blood of patients with bacterial DNA in their thrombotic material. Bacteria could be latently present in plaques and might play a role in plaque instability and thrombus formation leading to ACS.
Databáze: OpenAIRE
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