A Multimodality Image Guided Precision Radiation Research Platform: Integrating X-ray, Bioluminescence, and Fluorescence Tomography With Radiation Therapy
| Autor: | Anis Ahmad, F. Yang, Amir Keyvanloo, Thirupandiyur S. Udayakumar, Yidong Yang, Keying Xu, Junwei Shi |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Cancer Research Radiobiology medicine.medical_treatment Breast Neoplasms Radiation Dosage Multimodal Imaging Imaging phantom 030218 nuclear medicine & medical imaging 03 medical and health sciences Mice 0302 clinical medicine medicine Dosimetry Animals Radiology Nuclear Medicine and imaging Radiation treatment planning Radiometry Radiation Dosimeter business.industry Phantoms Imaging X-Ray Film Optical Imaging X-ray Prostatic Neoplasms Equipment Design Radiation therapy Benchmarking Oncology 030220 oncology & carcinogenesis Calibration Luminescent Measurements Female Tomography Nuclear medicine business Tomography X-Ray Computed Monte Carlo Method Neoplasm Transplantation Radiotherapy Image-Guided |
| Zdroj: | International journal of radiation oncology, biology, physics. 108(4) |
| ISSN: | 1879-355X |
| Popis: | Purpose Small animal irradiation is crucial to the investigation of radiobiological mechanisms. The paradigm of clinical radiation therapy is trending toward high-precision, stereotactic treatment. However, translating this scheme to small animal irradiation is challenging owing to the lack of high-quality image guidance. To overcome this obstacle, we developed a multimodality image guided precision radiation platform. Methods and Materials The platform consists of 4 modules: x-ray computed tomography (CT), bioluminescence tomography (BLT), fluorescence molecular tomography (FMT), and radiation therapy. CT provides animal anatomy and material density for radiation dose calculation, as well as body contour for BLT and FMT reconstruction. BLT and FMT provide tumor localization to guide radiation beams and molecular activity to evaluate treatment outcome. Furthermore, we developed a Monte Carlo–based treatment planning system (TPS) for 3-dimensional dose calculation, calibrated it using radiochromic films sandwiched in a water-equivalent phantom, and validated it using in vivo dosimeters surgically implanted into euthanized mice (n = 4). Finally, we performed image guided irradiation on mice bearing orthotopic breast and prostate tumors and confirmed radiation delivery using γH2AX histology. Results The Monte Carlo–based TPS was successfully calibrated by benchmarking simulation dose against film measurement. For in vivo dosimetry measured in the euthanized mice, the average difference between the TPS calculated dose and measured dose was 3.86% ± 1.12%. Following the TPS-generated treatment plan, we successfully delivered 20 Gy dose to an animal bearing an orthotopic prostate tumor using 4 BLT-guided radiation beams and 5 Gy dose to an animal bearing an orthotopic breast tumor using a single FMT-guided radiation beam. γH2AX histology presented significantly more DNA damage in irradiated tumors and thus validated the dose delivery accuracy. Conclusions Combined with Monte Carlo TPS, this multimodality CT/BLT/FMT image guided small animal radiation platform can specifically localize tumors, accurately calculate dose distribution, precisely guide radiation delivery, and molecularly evaluate treatment response. It provides an advanced toolset for radiobiology and translational cancer research. |
| Databáze: | OpenAIRE |
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