Labeling and qualification of endothelial progenitor cells for tracking in tissue engineering: An in vitro study
| Autor: | Alexandra Montembault, Noélie B. Thebaud, Jérôme Toutain, Robin Siadous, Laurence Bordenave, Laurent David, Reine Bareille, Audrey Aussel |
|---|---|
| Přispěvatelé: | Bioingénierie tissulaire (BIOTIS), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Bordeaux [Bordeaux], Ingénierie des Matériaux Polymères (IMP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Programmed cell death
Cell Biomedical Engineering Medicine (miscellaneous) Bioengineering 02 engineering and technology Lentiviral transduction Biology Bone and Bones Viral vector Biomaterials 03 medical and health sciences Transduction (genetics) Tissue engineering medicine Humans Progenitor cell Cell adhesion Endothelial progenitor cells 030304 developmental biology 0303 health sciences Staining and Labeling Tissue Scaffolds Labeled cells Osteoblast General Medicine [CHIM.MATE]Chemical Sciences/Material chemistry 021001 nanoscience & nanotechnology Fetal Blood Cell biology medicine.anatomical_structure Cell tracking [CHIM.POLY]Chemical Sciences/Polymers cardiovascular system Endothelium Vascular 0210 nano-technology Biomedical engineering |
| Zdroj: | International Journal of Artificial Organs International Journal of Artificial Organs, Wichtig Editore, 2015, 38, pp.224-232. ⟨10.5301/ijao.5000405⟩ |
| ISSN: | 0391-3988 |
| DOI: | 10.5301/ijao.5000405⟩ |
| Popis: | Purpose In order to track location and distribution of endothelial cells (ECs) within scaffolds in vitro, we chose lentiPGK-TdTomato transduction of human endothelial progenitor cells (EPCs) isolated and differentiated from cord blood. Because transduction could have a functional impact on cell behavior, we checked different parameters for qualification of labeled- EPCs as well as their use for potential applications in the context of vascular and bone tissue engineering. Methods After isolation and expansion, EPCs were classically characterized then transduced with the lentiviral vector containing the TdTomato protein gene under the control of the phosphoglycerate kinase (PGK) promoter. Conventional karyotyping, differentiation capacity, viability, proliferation assays were performed with labeled and unlabeled EPCs. Scaffolds and co-cultures were explored with labeled EPCs, in static or shear stress conditions. Results Our results show that cell labeling did not affect cell adhesion nor induce cell death. Cell labeling did not induce more chromosomal aberrations. Phenotypical characterization was not affected. In the context of tissue engineering applications, labeled EPCs maintained their ability to line 2D or 3D scaffolds, withstand physiological arterial shear stress, and form tubular networks in co-cultures with human osteoblast progenitor cells. Conclusions It is possible to label human EPCs with TdTomato without affecting their behavior by the transduction procedure. This creates an important tool for numerous applications. Our results provide a qualification of labeled EPCs in comparison with unlabeled ones for vascular and bone tissue engineering. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|