Transkingdom interactions between Lactobacilli and hepatic mitochondria attenuate western diet-induced diabetes
| Autor: | Richard R. Rodrigues, Christopher A. Gaulke, Briana Frink, Andrey Morgun, Franziska Bauchinger, Natalia Shulzhenko, Hyekyoung You, Thomas J. Sharpton, Zhipeng Li, Renee L. Greer, Giorgio Trinchieri, Stephany Vasquez-Perez, Jacob W. Pederson, Manuel García-Jaramillo, Benjamin Philmus, Amiran Dzutsev, Donald B. Jump, Manoj Gurung, David Berry, Kimberly D. White |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Science 030106 microbiology General Physics and Astronomy Mitochondria Liver Mitochondrion Gut flora Carbohydrate metabolism Article General Biochemistry Genetics and Molecular Biology law.invention 03 medical and health sciences Probiotic law Lactobacillus Animals Humans Metabolomics Obesity Microbiome Regulation of gene expression Multidisciplinary biology Reproducibility of Results Type 2 diabetes Bilirubin Lipid metabolism General Chemistry Lipid Metabolism biology.organism_classification Glutathione Gastrointestinal Microbiome Cell biology Mice Inbred C57BL Glucose 030104 developmental biology Diabetes Mellitus Type 2 Gene Expression Regulation Diet Western Transcriptome |
| Zdroj: | Nature Communications Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021) |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-020-20313-x |
| Popis: | Western diet (WD) is one of the major culprits of metabolic disease including type 2 diabetes (T2D) with gut microbiota playing an important role in modulating effects of the diet. Herein, we use a data-driven approach (Transkingdom Network analysis) to model host-microbiome interactions under WD to infer which members of microbiota contribute to the altered host metabolism. Interrogation of this network pointed to taxa with potential beneficial or harmful effects on host’s metabolism. We then validate the functional role of the predicted bacteria in regulating metabolism and show that they act via different host pathways. Our gene expression and electron microscopy studies show that two species from Lactobacillus genus act upon mitochondria in the liver leading to the improvement of lipid metabolism. Metabolomics analyses revealed that reduced glutathione may mediate these effects. Our study identifies potential probiotic strains for T2D and provides important insights into mechanisms of their action. Western diet is one of the major causes underlying diabetes, and the microbes residing in the gut playing a critical role in mediating the effects of diet. Here the authors utilize network analysis to discover two species of Lactobacilli decreased by western diet, which improve glucose metabolism and restore of hepatic mitochondria in mice. |
| Databáze: | OpenAIRE |
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