Effects of acth and cytokines on dehydroepiandrosterone sulfotransferase messenger RNA in human adrenal cells
| Autor: | Ana K. Stankovic, Ona Faye-Petersen, M. Jian, Charles N. Falany, H. Li, C. R. Parker |
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| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class Biology Inhibitory postsynaptic potential chemistry.chemical_compound Fetus Endocrinology Dehydroepiandrosterone sulfate Adrenocorticotropic Hormone Transforming Growth Factor beta Internal medicine Adrenal Glands medicine Humans RNA Messenger Gene Cells Cultured Messenger RNA Dose-Response Relationship Drug Tumor Necrosis Factor-alpha Steroid 17-alpha-Hydroxylase General Medicine Androgen chemistry Tumor necrosis factor alpha Sulfotransferases Transforming growth factor |
| Zdroj: | Endocrine Research. 24:669-673 |
| ISSN: | 1532-4206 0743-5800 |
| DOI: | 10.3109/07435809809032668 |
| Popis: | Dehydroepiandrosterone sulfate (DS) is the major adrenal androgen produced in the fetal and adult human; its formation is dependent upon the action of dehydroepiandrosterone sulfotransferase (DST). Since the factors that regulate DST are poorly characterized, we investigated the effects of ACTH, which stimulates DS production, and the cytokines transforming growth factor-beta (TGF-beta), and tumor necrosis factor-alpha (TNF-alpha) , both of which are inhibitory to adrenal steroidogenesis, on cultured human fetal adrenal cells. Cellular levels of DST mRNA were increased in a dose dependent fashion in response to ACTH; DST mRNA was less responsive to ACTH stimulation than was 17 hydroxylase (CYP 17) mRNA. The stimulatory effects of ACTH on DST mRNA levels were blunted by both TGF-beta and TNF-alpha; the inhibitory effects of TNF-alpha on DST mRNA were more striking than were those on CYP 17 mRNA. These data suggest that DS production can be altered by several agents acting on the DST gene. |
| Databáze: | OpenAIRE |
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