Quantifying Macrophages and Hemosiderin in Pediatric Dura Mater
| Autor: | Alison Krywanczyk, Elizabeth A. Bundock M.D. |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Pathology
medicine.medical_specialty Dura mater Iron Antigens Differentiation Myelomonocytic Autopsy Hemosiderin Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Hematoma Antigens CD Genetics medicine Humans 030216 legal & forensic medicine Forensic Pathology Staining and Labeling business.industry CD68 Macrophages Infant Newborn Subdural hemorrhage Infant Histology medicine.disease Immunohistochemistry medicine.anatomical_structure Child Preschool Dura Mater business 030217 neurology & neurosurgery Immunostaining |
| Zdroj: | Journal of forensic sciences. 63(3) |
| ISSN: | 1556-4029 |
| Popis: | Determining the age of a subdural hematoma at autopsy is of great interest for medicolegal purposes. The appearance of pigment-laden macrophages is often referenced as evidence that the subdural hematoma is 3-4 days old. However, understanding the significance of macrophages and hemosiderin requires understanding the histology of infant dura. Samples of grossly unremarkable dura taken from 17 pediatric autopsies were identified and histologically confirmed to lack subdural neomembrane. CD68 immunostaining and Prussian blue staining was performed. The CD68-positive cells per high-power field were quantified, and the presence of iron-containing cells was recorded. CD68-positive cells were present in all cases, even in the dural border layer. Iron-containing cells were identified in 59% of cases, and in the dural border layer in 29%. Therefore, CD68-positive and iron-containing cells can be present in pediatric dura without neomembrane or macroscopic subdural hemorrhage, and this requires consideration when estimating the age of a subdural hematoma. |
| Databáze: | OpenAIRE |
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