Effect of the spacer arm on non-specific binding in membrane affinity chromatography
| Autor: | Giulio Cesare Sarti, Eleonora Lalli, Cristiana Boi |
|---|---|
| Přispěvatelé: | Lalli, Eleonora, Sarti, Giulio C., Boi, Cristiana |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
membrane affinity spacer arm non-specific binding protein adsorption Materials science 010401 analytical chemistry 01 natural sciences Combinatorial chemistry 0104 chemical sciences Membrane Affinity chromatography Non specific 010608 biotechnology Deep knowledge Molecule General Materials Science Ligand molecule Protein adsorption |
| Zdroj: | MRS Communications. 8:65-70 |
| ISSN: | 2159-6867 2159-6859 |
| Popis: | The preparation, screening, and characterization of affinity membranes require a deep knowledge of the behavior of all components of the affinity material. Several studies report the effect of different spacers in combination with the ligand molecule, but the effect of the spacer arm “per se” is generally disregarded. The effect of the spacer 1,2-diaminoethane on non-specific protein adsorption was recently investigated and the results were compared with the ones obtained with A2P affinity membranes. The results show that this spacer has indeed an important effect and that similar specific studies need to be performed for every spacer molecule. |
| Databáze: | OpenAIRE |
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