Burkholderia pseudomalleiin soil samples from an oceanarium in Hong Kong detected using a sensitive PCR assay
| Autor: | Susanna K. P. Lau, San-Yuen Chan, Paolo Martelli, Suk-Wai Hui, Paul P. Lee, Chi-Chun Ho, Shirly O. T. Curreem, Patrick C. Y. Woo, Candy C. Y. Lau |
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| Rok vydání: | 2014 |
| Předmět: |
Burkholderia pseudomallei
Microbiological culture Melioidosis Soil test Epidemiology Immunology Pcr assay Biology Microbiology soil law.invention law Virology Drug Discovery medicine Polymerase chain reaction oceanarium General Medicine biology.organism_classification Isolation (microbiology) medicine.disease culture PCR Infectious Diseases Emerging infectious disease Original Article Parasitology melioidosis |
| Zdroj: | Emerging Microbes & Infections |
| ISSN: | 2222-1751 |
| DOI: | 10.1038/emi.2014.69 |
| Popis: | Melioidosis, caused by Burkholderia pseudomallei, is an emerging infectious disease with an expanding geographical distribution. Although assessment of the environmental load of B. pseudomallei is important for risk assessment in humans or animals in endemic areas, traditional methods of bacterial culture for isolation have low sensitivities and are labor-intensive. Using a specific polymerase chain reaction (PCR) assay targeting a Tat domain protein in comparison with a bacterial culture method, we examined the prevalence of B. pseudomallei in soil samples from an oceanarium in Hong Kong where captive marine mammals and birds have contracted melioidosis. Among 1420 soil samples collected from various sites in the oceanarium over a 15-month period, B. pseudomallei was detected in nine (0.6%) soil samples using bacterial culture, whereas it was detected in 96 (6.8%) soil samples using the specific PCR assay confirmed by sequencing. The PCR-positive samples were detected during various months, with higher detection rates observed during summer months. Positive PCR detection was significantly correlated with ambient temperature (P |
| Databáze: | OpenAIRE |
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