Evaluation of the genotoxicity in fish erythrocytes to diagnose the water quality of two Amazonian estuaries using the micronucleus test and comet assay

Autor: Bianca Bentes, Paulo Sergio dos Santos Souto, Tatiane do Nascimento Medeiros Rodrigues, Maria de Lourdes Souza Santos, Lorena Araújo da Cunha, Claudia Antonia Campos Rodrigues de OLiveira, Dulcideia da Conceição Palheta, Marcelo de Oliveira Bahia
Rok vydání: 2021
Předmět:
DOI: 10.21203/rs.3.rs-849055/v1
Popis: Genotoxicity studies in coastal ecosystems have been a priority in Environmental Risk Assessment (ERA). This research aimed to study the genotoxicity by the micronucleus test and comet assay in two Brazilian Amazon estuaries (anthropized and control) using Plagioscion squamosissimus as a bioindicator. Blood samples were collected from 54 specimens. No significant genotoxic effects were detected in the cells analyzed, although the highest occurrence was observed in anthropized site. The percentage of genomic damage differed between the sites studied, being always higher in anthropizes site as well. Of the nucleoids analyzed in this site, on average 28 ± 14.42% of the cells were classified in the highest damage class (4). The fish analyzed in the present study are direct influence of xenobiont agents capable of producing damage to the genetic material of aquatic organisms in both sites and, consequently, may bring consequences still little reported in studies of morphophysiological alterations in humans.
Databáze: OpenAIRE