Riboswitches that Sense S-adenosylhomocysteine and Activate Genes Involved in Coenzyme Recycling
| Autor: | Ronald R. Breaker, Joy Xin Wang, Elaine R. Lee, Jinsoo Lim, Dianali Rivera Morales |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Riboswitch
Untranslated region S-Adenosylmethionine Coenzymes Biology Article Conserved sequence Methionine Homocysteine Molecular Biology Gene Conserved Sequence Genetics Regulation of gene expression Binding Sites Base Sequence RNA Cell Biology S-Adenosylhomocysteine Kinetics RNA Bacterial Gene Expression Regulation Biochemistry Cobalamin riboswitch Nucleic Acid Conformation 5' Untranslated Regions Sequence motif |
| Zdroj: | Molecular Cell. 29:691-702 |
| ISSN: | 1097-2765 |
| DOI: | 10.1016/j.molcel.2008.01.012 |
| Popis: | We have identified a highly conserved RNA motif that occurs upstream of genes involved in S-adenosyl-l-methionine (SAM) recycling in many Gram-positive and Gram-negative species of bacteria. The phylogenetic distribution and the conserved structural features of representatives of this motif are indicative of riboswitch function. Riboswitches are widespread metabolite-sensing gene control elements that are typically found in the 5′ untranslated regions (UTRs) of bacterial mRNAs. We experimentally verified that examples of this RNA motif specifically recognize S-adenosylhomocysteine (SAH) in protein-free in vitro assays, and confirmed that these RNAs strongly discriminate against SAM and other closely related analogs. A representative SAH motif was found to activate expression of a downstream gene in vivo when the metabolite is bound. These observations confirm that SAH motif RNAs are distinct ligand-binding aptamers for a riboswitch class that selectively binds SAH and controls genes essential for recycling expended SAM coenzymes. |
| Databáze: | OpenAIRE |
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