Evaluation of a sex hormone-binding globulin automated chemiluminescent assay
Autor: | Massimo Gion, Silvia Michilin, Ruggero Dittadi, Aline S.C. Fabricio |
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Rok vydání: | 2013 |
Předmět: |
Adult
Male medicine.medical_specialty Globulin Serial dilution Clinical Biochemistry Hyperthyroidism law.invention Young Adult Sex hormone-binding globulin Hypothyroidism Pregnancy Reference Values law Sex Hormone-Binding Globulin Internal medicine medicine Humans reproductive and urinary physiology Aged Chemiluminescence Automation Laboratory medicine.diagnostic_test biology Chemistry Healthy subjects General Medicine Middle Aged Serum samples Postmenopause Endocrinology Premenopause Method comparison Immunoassay Luminescent Measurements biology.protein Female Blood Chemical Analysis hormones hormone substitutes and hormone antagonists |
Zdroj: | Scandinavian Journal of Clinical and Laboratory Investigation. 73:480-484 |
ISSN: | 1502-7686 0036-5513 |
Popis: | Sex hormone-binding globulin (SHBG) is the main transport protein of sex steroids. This study evaluated the analytical performance of the recently developed Access SHBG assay (Beckman Coulter) and compared it with other commercial methods for the determination of serum SHBG. Clinical validation was also performed.Analytical performance including within-run and between-run imprecision was assessed for Access SHBG assay on the automated Beckman UniCel DxI800 analyzer. Linearity was assessed using five dilutions of the serum samples. For methods comparison, SHBG levels were determined also with Immulite 2000 analyzer (Siemens Healthcare) using clinical serum samples (n = 104). For clinical validation 135 specimens from healthy subjects, pregnant women, hypothyroid and hyperthyroid patients were analyzed.Total coefficients of variation were5.5%. Linearity test showed90% recovery for all samples and for all dilution rates. Comparison analysis (Bland-Altman difference analysis and Passing-Bablock regression) showed an acceptable agreement between selected methods. SHBG values measured by Access SHBG assay in different groups of subjects were in agreement with other clinical evidence.Automated Access SHBG assay appears to be a reliable and easy to perform assay, as necessary for application in routine diagnostics. |
Databáze: | OpenAIRE |
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