Altered mitochondrial apparent affinity for ADP and impaired function of mitochondrial creatine kinase in gluteus medius of patients with hip osteoarthritis
| Autor: | Teet Seene, Enn Seppet, Anneli Sikkut, Tiit Haviko, Nadezhda Peet, Evelin Seppet, Margus Eimre, Lumme Kadaja, Raivo Puhke, Karin Alev, Valdur Saks, Aleks Lenzner |
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| Přispěvatelé: | Department of Pathophysiology, University of Tartu, Department of Functional Morphology, Department of Traumatology and Orthopaedics, Laboratory of Bioenergetics, National Institute of Chemical Physics and Biophysics = Keemilise ja bioloogilise füüsika instituut [Estonie] (NICPB | KBFI), Laboratoire de bioénergétique fondamentale et appliquée (LBFA), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hamant, Sarah |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Male
Physiology MESH: Muscle Smooth MESH: Myosin Heavy Chains Osteoarthritis Hip Oxidative Phosphorylation 0302 clinical medicine Myosin Creatine Kinase MESH: Aged 0303 health sciences MESH: Creatine Kinase MESH: Middle Aged biology MESH: Energy Metabolism Middle Aged Mitochondria Adenosine Diphosphate Medius Biochemistry Musculus gluteus medius Female medicine.medical_specialty MESH: Mitochondria Oxidative phosphorylation 03 medical and health sciences MESH: Oxidative Phosphorylation Physiology (medical) Internal medicine [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Hip osteoarthritis medicine Humans [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology 030304 developmental biology Aged MESH: Humans Myosin Heavy Chains MESH: Adenosine Diphosphate Muscle Smooth Metabolism biology.organism_classification MESH: Male Endocrinology biology.protein Creatine kinase MESH: Osteoarthritis Hip Energy Metabolism MESH: Female 030217 neurology & neurosurgery Function (biology) |
| Zdroj: | AJP-Regulatory, Integrative and Comparative Physiology AJP-Regulatory, Integrative and Comparative Physiology, American Physiological Society, 2006, 290 (5), pp.R1271-5. ⟨10.1152/ajpregu.00651.2005⟩ |
| ISSN: | 0363-6119 1522-1490 |
| Popis: | The cellular energy metabolism in human musculus gluteus medius (MGM) under normal conditions and hip osteoarthritis (OA) was explored. The functions of oxidative phosphorylation and energy transport systems were analyzed in permeabilized (skinned) muscle fibers by oxygraphy, in relation to myosin heavy chain (MHC) isoform distribution profile analyzed by SDS-PAGE, and to creatine kinase (CK) and adenylate kinase (AK) activities measured spectrophotometrically in the intact muscle. The results revealed high apparent Km for ADP in regulation of respiration that decreased after addition of creatine in MGM of traumatic patients (controls). OA was associated with increased sensitivity of mitochondrial respiration to ADP, decreased total activities of AK and CK with major reduction in mi-CK fraction, and attenuated effect of creatine on apparent Km for ADP compared with control group. It also included a complete loss of type II fibers in a subgroup of patients with the severest disease grade. It is concluded that energy metabolism in MGM cells is organized into functional complexes of mitochondria and ATPases. It is suggested that because of degenerative remodeling occurring during development of OA, these complexes become structurally and functionally impaired, which results in increased access of exogenous ADP to mitochondria and dysfunction of CK-phosphotransfer system. |
| Databáze: | OpenAIRE |
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