Effect of the Aspect Ratio of Coiled-Coil Protein Carriers on Cellular Uptake
| Autor: | Megumi Ota, Kentaro Takagaki, Ken-Ichi Sano, Sho Takaoka, Norihisa Nakayama |
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| Rok vydání: | 2018 |
| Předmět: |
Models
Molecular Aspect ratio Peptide 02 engineering and technology Cell-Penetrating Peptides 010402 general chemistry 01 natural sciences Protein Structure Secondary Nanomaterials Protein structure Electrochemistry Humans General Materials Science Amino Acid Sequence Peptide sequence Spectroscopy chemistry.chemical_classification Cationic polymerization Biological Transport Surfaces and Interfaces Polymer 021001 nanoscience & nanotechnology Condensed Matter Physics 0104 chemical sciences chemistry A549 Cells Biophysics Nanorod 0210 nano-technology |
| Zdroj: | Langmuir : the ACS journal of surfaces and colloids. 34(47) |
| ISSN: | 1520-5827 |
| Popis: | We showed previously that a rigid and fibrous-structured cationic coiled-coil artificial protein had cell-penetrating activity that was significantly greater when compared with a less-structured cell-penetrating peptide. Nanomaterials with anisotropic structures often show aspect-ratio-dependent unique physicochemical properties, as well as cell-penetrating activities. In this report, we have designed and demonstrated the cell-penetrating activity of a shorter cationic coiled-coil protein. An aspect ratio at 4.5:1 was found to be critical for ensuring that the cationic coiled-coil protein showed strong cell-penetrating activity. At an aspect ratio of 3.5:1, the cationic coiled-coil protein showed cell-penetrating activity that was similar to a less-structured short cationic cell-penetrating peptide. Interestingly, at an aspect ratio of 4:1, the cationic coiled-coil protein exhibited intermediate cell-penetrating activity. These findings should aid in the principle design of intracellular drug delivery carriers including coiled-coil artificial proteins, their derivatives, and α-helical cell-penetrating peptides as well as provide a framework for developing synthetic nanomaterials, such as metal nanorods and synthetic polymers. |
| Databáze: | OpenAIRE |
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